Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 57127..57652 Replicon plasmid unnamed2
Accession NZ_CP029740
Organism Klebsiella pneumoniae strain AR_0087

Toxin (Protein)


Gene name ccdB Uniprot ID D4HQE7
Locus tag AM466_RS28495 Protein ID WP_013023785.1
Coordinates 57127..57432 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag AM466_RS28500 Protein ID WP_001568025.1
Coordinates 57434..57652 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM466_RS28465 52822..53448 + 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -
AM466_RS30030 53445..53747 + 303 WP_071571079.1 hypothetical protein -
AM466_RS28475 54203..54997 - 795 WP_004197635.1 site-specific integrase -
AM466_RS28480 55195..56211 - 1017 WP_017899884.1 hypothetical protein -
AM466_RS28485 56222..56536 - 315 WP_053389906.1 hypothetical protein -
AM466_RS28490 56563..56958 - 396 WP_017899885.1 hypothetical protein -
AM466_RS28495 57127..57432 - 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB Toxin
AM466_RS28500 57434..57652 - 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
AM466_RS28510 57822..58283 - 462 Protein_79 hypothetical protein -
AM466_RS28515 58240..58470 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
AM466_RS28520 58467..58883 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin -
AM466_RS28525 58957..59667 + 711 WP_004152334.1 ATP-binding protein -
AM466_RS28530 59701..60398 + 698 Protein_83 IS1 family transposase -
AM466_RS28535 60399..61247 + 849 WP_048322264.1 SDR family oxidoreductase -
AM466_RS28540 61339..61983 - 645 WP_014386481.1 quinolone resistance pentapeptide repeat protein QnrB1 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid qnrB1 / blaSHV-12 / tet(A) - 1..103250 103250


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11615.30 Da        Isoelectric Point: 6.4672

>T106594 WP_013023785.1 NZ_CP029740:c57432-57127 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T106594 NZ_CP029740:c57432-57127 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT106594 WP_001568025.1 NZ_CP029740:c57652-57434 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT106594 NZ_CP029740:c57652-57434 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4ZZW6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References