Detailed information of TA system TA106580

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 3195890..3196515
• Replicon: chromosome
• Accession: NZ_CP029738
• Organism: Klebsiella pneumoniae strain AR_0087

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: AM466_RS16145
• Protein ID: WP_040182550.1
• Coordinates: 3195890..3196273 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: J2DFR0
• Locus tag: AM466_RS16150
• Protein ID: WP_004150355.1
• Coordinates: 3196273..3196515 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM466_RS16130	3193256..3194158	+	903	WP_002882822.1	formate dehydrogenase subunit beta	-
AM466_RS16135	3194155..3194790	+	636	WP_002882818.1	formate dehydrogenase cytochrome b556 subunit	-
AM466_RS16140	3194787..3195716	+	930	WP_004150358.1	formate dehydrogenase accessory protein FdhE	-
AM466_RS16145	3195890..3196273	-	384	WP_040182550.1	type II toxin-antitoxin system VapC family toxin	Toxin
AM466_RS16150	3196273..3196515	-	243	WP_004150355.1	ribbon-helix-helix domain-containing protein	Antitoxin
AM466_RS16155	3196720..3197637	+	918	WP_023302328.1	alpha/beta hydrolase	-
AM466_RS16160	3197651..3198592	-	942	WP_004178031.1	fatty acid biosynthesis protein FabY	-
AM466_RS16165	3198637..3199074	-	438	WP_002882809.1	D-tyrosyl-tRNA(Tyr) deacylase	-
AM466_RS16170	3199071..3199931	-	861	WP_004146232.1	virulence factor BrkB family protein	-
AM466_RS16175	3199925..3200524	-	600	WP_004151865.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-112)

Antitoxin

• Predicted DNA-binding protein, ribbon-helix-helix domain (16-47)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14376.67 Da
• Isoelectric Point: 9.5352

>T106580 WP_040182550.1 NZ_CP029738:c3196273-3195890 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLKLITRNTKDFAGIPGVVTPYEIHPE

Nucleotide

• Length: 384 bp

>T106580 NZ_CP029738:c3196273-3195890 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGTACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGAAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9567.83 Da
• Isoelectric Point: 4.3509

>AT106580 WP_004150355.1 NZ_CP029738:c3196515-3196273 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Nucleotide

• Length: 243 bp

>AT106580 NZ_CP029738:c3196515-3196273 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GGU9