Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 95881..96406 Replicon plasmid unnamed3
Accession NZ_CP029731
Organism Citrobacter sp. CRE-46 strain AR_0157

Toxin (Protein)


Gene name ccdB Uniprot ID W8EAA6
Locus tag AN232_RS29385 Protein ID WP_008322213.1
Coordinates 95881..96186 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A6C0NE25
Locus tag AN232_RS29390 Protein ID WP_032934863.1
Coordinates 96188..96406 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AN232_RS29345 90982..91134 + 153 Protein_103 IS5/IS1182 family transposase -
AN232_RS29355 91206..92326 + 1121 WP_089046468.1 IS3 family transposase -
AN232_RS29360 92355..93101 + 747 Protein_105 IS5 family transposase -
AN232_RS29370 93263..94585 + 1323 WP_016479957.1 GntP family transporter -
AN232_RS29375 94784..95575 - 792 WP_008322208.1 site-specific integrase -
AN232_RS29380 95565..95879 - 315 WP_008322211.1 hypothetical protein -
AN232_RS29385 95881..96186 - 306 WP_008322213.1 type II toxin-antitoxin system toxin CcdB Toxin
AN232_RS29390 96188..96406 - 219 WP_032934863.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
AN232_RS29395 97268..97965 - 698 Protein_111 IS1 family transposase -
AN232_RS29405 98022..98222 + 201 Protein_112 IS30 family transposase -
AN232_RS29410 98323..99135 + 813 WP_004201164.1 subclass B1 metallo-beta-lactamase NDM-1 -
AN232_RS29415 99139..99504 + 366 WP_004201167.1 bleomycin binding protein Ble-MBL -
AN232_RS29420 99509..100147 + 639 WP_004201168.1 phosphoribosylanthranilate isomerase -
AN232_RS29425 100158..101189 - 1032 WP_004201169.1 protein-disulfide reductase DsbD N-terminal domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul1 / rmtC / blaNDM-1 htpB 1..108148 108148
- inside IScluster/Tn rmtC / blaNDM-1 - 83436..99135 15699


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11450.23 Da        Isoelectric Point: 5.8211

>T106565 WP_008322213.1 NZ_CP029731:c96186-95881 [Citrobacter sp. CRE-46]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLVSARLLSDKVSRELYPVVSVGGDPYRLMTTDMASVPATVIGEEV
ADLSHLENDIQNAINLMFRGI

Download         Length: 306 bp

>T106565 NZ_CP029731:c96186-95881 [Citrobacter sp. CRE-46]
ATGCAGTTTAAGGTTTACGCCTATAAAAGAGAAAGCCGCTACCGTCTGTTCGTCGACGTGCAGAGTGACATTATTGACAC
CCCGGGACGCCGGATGGTGATCCCGCTGGTCAGCGCCCGCCTGCTGTCCGACAAAGTCTCGCGTGAGCTTTATCCGGTGG
TGTCAGTCGGAGGTGACCCTTATCGTCTGATGACCACGGATATGGCCAGTGTGCCGGCCACTGTCATCGGGGAAGAAGTG
GCCGATCTCAGCCACCTGGAGAATGACATCCAGAACGCCATCAACCTGATGTTCCGGGGGATCTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8297.11 Da        Isoelectric Point: 4.4013

>AT106565 WP_032934863.1 NZ_CP029731:c96406-96188 [Citrobacter sp. CRE-46]
VKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQEENREGMAEVASFIEANGSFADENRDW

Download         Length: 219 bp

>AT106565 NZ_CP029731:c96406-96188 [Citrobacter sp. CRE-46]
GTGAAGCAGCGTATTACAGTGACAGTGGACAGCGACAGCTATCAGTTGCTCAAGGCATACGACGTAAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGCCGTCTGCGCGCCGAACGCTGGCAGGAAGAAAACCGGGAAGGTATGGCTG
AGGTGGCCAGCTTTATCGAGGCTAACGGATCGTTTGCTGACGAAAACAGGGATTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6C0NE27

Antitoxin

Source ID Structure
AlphaFold DB A0A6C0NE25

References