Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/ElaA-DUF1778
Location 86817..87620 Replicon plasmid unnamed3
Accession NZ_CP029731
Organism Citrobacter sp. CRE-46 strain AR_0157

Toxin (Protein)


Gene name KacT Uniprot ID W8E895
Locus tag AN232_RS29325 Protein ID WP_016479963.1
Coordinates 86817..87347 (-) Length 177 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID W8E6Q5
Locus tag AN232_RS29330 Protein ID WP_022652312.1
Coordinates 87351..87620 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AN232_RS29300 83436..84281 + 846 WP_000855769.1 RmtC family 16S rRNA (guanine(1405)-N(7))-methyltransferase -
AN232_RS29305 84644..84763 + 120 Protein_96 IS30 family transposase -
AN232_RS29310 84773..85522 + 750 Protein_97 IS3 family transposase -
AN232_RS29315 85669..86685 - 1017 WP_087728544.1 IS5-like element IS5 family transposase -
AN232_RS29325 86817..87347 - 531 WP_016479963.1 GNAT family N-acetyltransferase Toxin
AN232_RS29330 87351..87620 - 270 WP_022652312.1 DUF1778 domain-containing protein Antitoxin
AN232_RS29335 88476..89465 + 990 WP_022652311.1 RepB family plasmid replication initiator protein -
AN232_RS29340 89761..90500 - 740 Protein_102 site-specific integrase -
AN232_RS29345 90982..91134 + 153 Protein_103 IS5/IS1182 family transposase -
AN232_RS29355 91206..92326 + 1121 WP_089046468.1 IS3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul1 / rmtC / blaNDM-1 htpB 1..108148 108148
- inside IScluster/Tn rmtC / blaNDM-1 - 83436..99135 15699


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(92-156)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 177 a.a.        Molecular weight: 20350.24 Da        Isoelectric Point: 6.4907

>T106564 WP_016479963.1 NZ_CP029731:c87347-86817 [Citrobacter sp. CRE-46]
MEGLRIEIFSEEVEYELSNFDCGEEYLNTFLTDHLKRQHNSKILRGYVLVTREDKPRVMGYYTLSGSCFEKILLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENCNSL
FYPTKSIEELFEVNDE

Download         Length: 531 bp

>T106564 NZ_CP029731:c87347-86817 [Citrobacter sp. CRE-46]
ATGGAGGGTTTAAGGATTGAGATTTTTTCAGAGGAAGTTGAATATGAATTAAGCAACTTCGATTGTGGAGAAGAGTATCT
GAATACTTTTCTTACTGACCATCTGAAACGGCAGCATAACAGTAAGATCTTACGTGGTTATGTTCTCGTGACGAGAGAGG
ATAAACCGCGTGTGATGGGGTACTACACGCTATCAGGAAGTTGCTTTGAGAAAATACTTCTACCCAGCAAAACACAACAA
AAGCGTGTACCCTACAAGAATGTGCCGAGCGTGACTTTGGGCCGTCTGGCAATTGATAAAAGCATTCACCACCAGGGATA
TGGAGAAACACTTGTTACCCATGCGATGAAAGTCGTGTATCAGGCCTCGCAGGCTGTGGGTATACACGGAATGTTCGTGG
AAGCGCTGAACGACAACGCCAAAAAATTCTACCTTCGACTGGGTTTCATTCAGTTAAAGGAAGAAAACTGTAACTCGCTC
TTTTACCCGACAAAATCTATTGAAGAATTGTTCGAAGTAAATGATGAATAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10269.70 Da        Isoelectric Point: 5.7252

>AT106564 WP_022652312.1 NZ_CP029731:c87620-87351 [Citrobacter sp. CRE-46]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASERAAEVIDQHRRLLLNEESWNLVMDAIINPPAPNDRLKR
AANRLRELE

Download         Length: 270 bp

>AT106564 NZ_CP029731:c87620-87351 [Citrobacter sp. CRE-46]
ATGTCAGCACTGAAAAAGCAGCGCATTGACCTGAGATTAAATGAAGACGACAAACACATGATCGAAGAAGCTGCAGCAAT
GACTAACCAGTCAATTTCGCAGTTCATGGTCAGTACCGCCTCAGAACGCGCTGCAGAAGTTATTGACCAGCACCGTCGTC
TGCTCCTCAATGAGGAGTCTTGGAACCTGGTTATGGATGCAATCATTAATCCGCCAGCACCGAATGACCGGCTGAAACGC
GCCGCTAACCGTCTCCGGGAACTGGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6C0L7Q2

Antitoxin

Source ID Structure
AlphaFold DB A0A2Z3XDS5

References