106562

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/MqsA(antitoxin)
Location 15808..16273 Replicon plasmid unnamed8
Accession NZ_CP029728
Organism Citrobacter sp. CRE-46 strain AR_0157

Toxin (Protein)


Gene name TsxA Uniprot ID U9ZUY2
Locus tag AN232_RS26415 Protein ID WP_001230707.1
Coordinates 16022..16273 (-) Length 84 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID M7CCF8
Locus tag AN232_RS26410 Protein ID WP_000801440.1
Coordinates 15808..16035 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AN232_RS26360 11038..11253 - 216 WP_000760375.1 EexN family lipoprotein -
AN232_RS26365 11250..11543 - 294 WP_000134733.1 LuxR family transcriptional regulator -
AN232_RS26370 11620..12849 - 1230 WP_001329504.1 relaxase/mobilization nuclease domain-containing protein -
AN232_RS26375 12853..13356 - 504 WP_000781813.1 hypothetical protein -
AN232_RS26385 13717..14064 - 348 WP_000681613.1 type II toxin-antitoxin system HicB family antitoxin -
AN232_RS26390 14061..14309 - 249 WP_000520549.1 type II toxin-antitoxin system HicA family toxin -
AN232_RS26395 14614..14964 - 351 WP_000854259.1 hypothetical protein -
AN232_RS26400 15055..15354 - 300 WP_162620745.1 hypothetical protein -
AN232_RS26410 15808..16035 - 228 WP_000801440.1 hypothetical protein Antitoxin
AN232_RS26415 16022..16273 - 252 WP_001230707.1 hypothetical protein Toxin
AN232_RS26420 16270..16812 - 543 WP_001083906.1 hypothetical protein -
AN232_RS31525 16917..17432 - 516 WP_001025395.1 J domain-containing protein -
AN232_RS26430 18847..19713 + 867 WP_001353743.1 replication initiation protein -
AN232_RS26435 19726..20130 + 405 WP_012421203.1 hypothetical protein -
AN232_RS26440 20265..20792 + 528 WP_000577043.1 DUF2726 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..31764 31764


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Protein of unknown function DUF1474

(1-97)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 84 a.a.        Molecular weight: 9751.13 Da        Isoelectric Point: 8.9187

>T106562 WP_001230707.1 NZ_CP029728:c16273-16022 [Citrobacter sp. CRE-46]
MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDAVLNENELQHIDFTCAGLREDDETPVKNLIKEFMLN
ETR

Download         Length: 252 bp

>T106562 NZ_CP029728:c16273-16022 [Citrobacter sp. CRE-46]
ATGAGAAAAAATAAAGGTGATGTGACTTATTTTCTTGAAAAAGAAGGTGACAATTACAGGCTTACAAAAAGGATAAAAGC
CAGAACAAATGTTAAAATCGGGAACAAAACTACGAAAATTACATTGTATGATGCTGTACTGAATGAGAATGAATTACAAC
ATATTGATTTTACGTGTGCCGGATTAAGGGAAGACGATGAGACGCCAGTTAAGAATTTAATTAAGGAGTTTATGTTAAAT
GAAACCCGATAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8659.98 Da        Isoelectric Point: 8.5105

>AT106562 WP_000801440.1 NZ_CP029728:c16035-15808 [Citrobacter sp. CRE-46]
MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQNSQSLSLVEFEFLLLLAGEHPEYVLCKRESK

Download         Length: 228 bp

>AT106562 NZ_CP029728:c16035-15808 [Citrobacter sp. CRE-46]
ATGAAACCCGATAATACACCTGAAAATGTAAAAAAATTACGTTTAAAAGCAGGATTAACACAAAAGGAATGCAGTCATAT
TTATGGTGTTGGGCTAAGAACCTGGCAGAAAAAAGAGGAAGTTAATACTCAAAACAGTCAAAGTCTGTCACTGGTTGAGT
TTGAGTTTCTTTTGTTACTTGCAGGAGAACATCCTGAATACGTGCTCTGCAAAAGGGAGTCAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9ZUY2

Antitoxin

Source ID Structure
AlphaFold DB A0A5Y6U8W9

References