Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 13717..14309 Replicon plasmid unnamed8
Accession NZ_CP029728
Organism Citrobacter sp. CRE-46 strain AR_0157

Toxin (Protein)


Gene name hicA Uniprot ID V0AGQ2
Locus tag AN232_RS26390 Protein ID WP_000520549.1
Coordinates 14061..14309 (-) Length 83 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID V0AWG0
Locus tag AN232_RS26385 Protein ID WP_000681613.1
Coordinates 13717..14064 (-) Length 116 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AN232_RS26350 9721..9993 - 273 WP_001328551.1 TrbC/VirB2 family protein -
AN232_RS26355 10061..10681 - 621 WP_000157549.1 lytic transglycosylase domain-containing protein -
AN232_RS26360 11038..11253 - 216 WP_000760375.1 EexN family lipoprotein -
AN232_RS26365 11250..11543 - 294 WP_000134733.1 LuxR family transcriptional regulator -
AN232_RS26370 11620..12849 - 1230 WP_001329504.1 relaxase/mobilization nuclease domain-containing protein -
AN232_RS26375 12853..13356 - 504 WP_000781813.1 hypothetical protein -
AN232_RS26385 13717..14064 - 348 WP_000681613.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
AN232_RS26390 14061..14309 - 249 WP_000520549.1 type II toxin-antitoxin system HicA family toxin Toxin
AN232_RS26395 14614..14964 - 351 WP_000854259.1 hypothetical protein -
AN232_RS26400 15055..15354 - 300 WP_162620745.1 hypothetical protein -
AN232_RS26410 15808..16035 - 228 WP_000801440.1 hypothetical protein -
AN232_RS26415 16022..16273 - 252 WP_001230707.1 hypothetical protein -
AN232_RS26420 16270..16812 - 543 WP_001083906.1 hypothetical protein -
AN232_RS31525 16917..17432 - 516 WP_001025395.1 J domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..31764 31764


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(20-81)

Antitoxin

Uncharacterised protein family HicB

(54-104)


Sequences

Toxin        


Download         Length: 83 a.a.        Molecular weight: 9422.08 Da        Isoelectric Point: 10.4800

>T106561 WP_000520549.1 NZ_CP029728:c14309-14061 [Citrobacter sp. CRE-46]
MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
LL

Download         Length: 249 bp

>T106561 NZ_CP029728:c14309-14061 [Citrobacter sp. CRE-46]
ATGGGGAAAACAGATAAGCTACTGGCAAAGTTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTGGTCGTTTTGTT
TTCCTCTTTGGGATATGTCAAAAAGGAAATGCAGGGGTCAAGAGTGCGGTTTTTCAATGCTGAAATCAATCACACCATAT
TAATGCATCGTCCACATCCGGAAAGTTATATCAAAGGTGGTACGCTGAAAGCAATTAAACAGAATCTGAAAGAGGCTGGA
TTATTATGA

Antitoxin


Download         Length: 116 a.a.        Molecular weight: 13113.84 Da        Isoelectric Point: 4.6723

>AT106561 WP_000681613.1 NZ_CP029728:c14064-13717 [Citrobacter sp. CRE-46]
MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQEFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSNHRAGA

Download         Length: 348 bp

>AT106561 NZ_CP029728:c14064-13717 [Citrobacter sp. CRE-46]
ATGAAACATTTAAAATATAAAGGATATTTAGGCACGGTTGAACCCGATTTTGAAAATAATGTCCTGTATGGAAAACTGGC
GTTTATTCGGGACCTGGTAACTTATGAGGCTTCTACATTAGCTGAACTGGAACAGGAGTTTAAAACATCTGTTGATCTGT
ATTTACAGTCTTGTGTGGAGGATGGAAAGGAACCTGATACCCCGTTTAAAGGCGTGTTTAACGTCAGGCTTGATCCAGAA
CTGCATCGTCGGGTAGCAGAAATGGCGATGGAAGAAGATTTATCGTTGAATGCCTTTGTTAATAAGGCTCTGGAAAAAGA
AGTCAGCAATCATCGCGCAGGGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CIX3

Antitoxin

Source ID Structure
AlphaFold DB A0A829CLC1

References