Detailed information of TA system TA106558

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 3541593..3542232
• Replicon: chromosome
• Accession: NZ_CP029727
• Organism: Citrobacter sp. CRE-46 strain AR_0157

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: AN232_RS17590
• Protein ID: WP_085048165.1
• Coordinates: 3541593..3541769 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: AN232_RS17595
• Protein ID: WP_162620740.1
• Coordinates: 3541816..3542232 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AN232_RS17570	3538370..3538600	-	231	WP_038640744.1	DUF2554 family protein	-
AN232_RS17575	3538790..3538915	-	126	WP_064507023.1	DUF2474 domain-containing protein	-
AN232_RS17580	3538915..3539925	-	1011	WP_085048163.1	cytochrome d ubiquinol oxidase subunit II	-
AN232_RS17585	3539925..3541328	-	1404	WP_110498018.1	cytochrome ubiquinol oxidase subunit I	-
AN232_RS17590	3541593..3541769	+	177	WP_085048165.1	type II toxin-antitoxin system HicA family toxin	Toxin
AN232_RS17595	3541816..3542232	+	417	WP_162620740.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
AN232_RS17600	3542325..3543734	+	1410	WP_069324935.1	PLP-dependent aminotransferase family protein	-
AN232_RS17605	3544064..3545209	+	1146	WP_110498022.1	ABC transporter substrate-binding protein	-
AN232_RS17610	3545226..3546242	+	1017	WP_110498024.1	ABC transporter ATP-binding protein	-
AN232_RS17615	3546243..3547187	+	945	WP_069324933.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-66)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6783.83 Da
• Isoelectric Point: 11.6208

>T106558 WP_085048165.1 NZ_CP029727:3541593-3541769 [Citrobacter sp. CRE-46]
VKQSEFRRWLESQGVKVTNGSNHLKLRYQGRRSVMPRHPGDEIKEALRKAIQKQLGLQ

Nucleotide

• Length: 177 bp

>T106558 NZ_CP029727:3541593-3541769 [Citrobacter sp. CRE-46]
GTGAAGCAAAGTGAGTTCAGACGCTGGCTTGAATCTCAGGGAGTTAAAGTTACAAACGGCTCAAATCATCTGAAGTTGAG
GTATCAGGGAAGGCGTAGCGTTATGCCCAGGCATCCTGGTGATGAGATAAAAGAGGCCCTGCGGAAAGCGATCCAAAAGC
AGCTTGGCTTACAGTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15036.30 Da
• Isoelectric Point: 4.5468

>AT106558 WP_162620740.1 NZ_CP029727:3541816-3542232 [Citrobacter sp. CRE-46]
MRYPVNLIPAVEGGFMVSFPDIPEALTQGETRHDALQAAQAALVTAFEFYFDDNEPIPLPSAVAAGADYVEIPLSVASKV
LLLNAFLESNITQQELANRIGRPKQEITRLFDLKHATKIDAVQIAARALGKELSVTML

Nucleotide

• Length: 417 bp

>AT106558 NZ_CP029727:3541816-3542232 [Citrobacter sp. CRE-46]
ATGCGTTATCCCGTTAATCTAATACCCGCCGTGGAAGGTGGGTTCATGGTGTCTTTTCCGGATATTCCTGAAGCATTGAC
TCAGGGAGAAACTCGCCATGATGCTTTGCAGGCTGCGCAGGCCGCATTGGTCACCGCTTTTGAATTCTATTTCGACGATA
ACGAACCGATTCCTTTGCCTTCAGCGGTTGCCGCTGGCGCTGACTATGTGGAAATACCACTGAGCGTTGCGTCTAAGGTT
CTGTTGTTAAATGCCTTCCTTGAATCGAATATTACCCAGCAGGAGTTGGCAAACAGGATCGGCAGGCCTAAGCAGGAAAT
CACCCGTTTATTCGATTTAAAGCACGCGACAAAAATTGATGCTGTGCAGATAGCTGCCCGCGCTTTAGGAAAAGAACTGT
CAGTAACGATGTTGTAA