Detailed information of TA system TA106548

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 1166940..1167571
• Replicon: chromosome
• Accession: NZ_CP029727
• Organism: Citrobacter sp. CRE-46 strain AR_0157

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0J1MPQ6
• Locus tag: AN232_RS05815
• Protein ID: WP_003837806.1
• Coordinates: 1166940..1167215 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: AN232_RS05820
• Protein ID: WP_110496595.1
• Coordinates: 1167212..1167571 (+)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AN232_RS05805	1162991..1165738	+	2748	WP_110496593.1	ribosome-associated ATPase/putative transporter RbbA	-
AN232_RS05810	1165738..1166862	+	1125	WP_038635040.1	ABC transporter permease	-
AN232_RS05815	1166940..1167215	+	276	WP_003837806.1	type II toxin-antitoxin system HicA family toxin	Toxin
AN232_RS05820	1167212..1167571	+	360	WP_110496595.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
AN232_RS05825	1167638..1168039	-	402	WP_006687486.1	nickel-responsive transcriptional regulator NikR	-
AN232_RS05830	1168131..1168991	-	861	WP_110496597.1	LysR family transcriptional regulator	-
AN232_RS05835	1169091..1169597	+	507	WP_110496599.1	phenolic acid decarboxylase	-
AN232_RS05840	1169589..1170179	-	591	WP_038635032.1	4'-phosphopantetheinyl transferase AcpT	-
AN232_RS05845	1170242..1171291	-	1050	WP_032940622.1	AI-2E family transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-80)

Antitoxin

• Uncharacterised protein family HicB (60-112)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10210.88 Da
• Isoelectric Point: 10.7228

>T106548 WP_003837806.1 NZ_CP029727:1166940-1167215 [Citrobacter sp. CRE-46]
MKEKVLSLRKKQKNTLDQLFKAPTPQGIKWSEIESLIKALGGEIKEGRGSRCKFLLNKSIASFHRPHPSPDTDKGAVENV
RDWLTSIGVKP

Nucleotide

• Length: 276 bp

>T106548 NZ_CP029727:1166940-1167215 [Citrobacter sp. CRE-46]
ATGAAGGAAAAGGTGTTGTCTTTACGCAAAAAGCAAAAGAACACGCTGGATCAGCTTTTTAAAGCGCCTACGCCGCAGGG
AATCAAGTGGTCGGAAATCGAGTCACTGATTAAGGCATTAGGTGGTGAAATCAAAGAAGGGCGAGGTTCGCGATGTAAAT
TTCTTTTAAATAAGAGCATTGCGAGTTTTCATCGACCACATCCTTCTCCGGATACAGATAAAGGCGCGGTCGAAAACGTA
CGTGACTGGTTAACAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13222.06 Da
• Isoelectric Point: 4.7022

>AT106548 WP_110496595.1 NZ_CP029727:1167212-1167571 [Citrobacter sp. CRE-46]
MIKPKTLNSMEIAGQPAVINYVPELNAFRGKFLGLSGYCDFVADSIQGLQKEGEISLREYLDDCSAAGIEPYAHPEKLKT
FTLRYPESFGERLSFAAAEEQVSVNTWIIETLSKSLKQA

Nucleotide

• Length: 360 bp

>AT106548 NZ_CP029727:1167212-1167571 [Citrobacter sp. CRE-46]
ATGATCAAACCCAAAACGCTAAACTCAATGGAAATCGCTGGGCAACCTGCGGTGATTAATTATGTCCCTGAGCTTAATGC
GTTCCGCGGTAAGTTCCTGGGTTTATCAGGCTATTGTGATTTTGTTGCTGACAGCATTCAGGGGCTGCAAAAAGAGGGGG
AGATTTCCTTACGCGAGTATCTGGATGATTGCTCTGCCGCAGGGATAGAGCCCTATGCTCACCCGGAAAAGCTGAAGACA
TTTACGCTACGCTACCCAGAGTCATTCGGAGAACGTTTAAGTTTTGCGGCAGCGGAAGAACAGGTATCAGTGAATACCTG
GATTATCGAAACGCTTAGTAAAAGCCTGAAACAGGCATAG

Structures

Toxin

• AlphaFold DB: A0A0J1MPQ6