106543

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) ykfI-yfjZ/CbtA-CbeA
Location 147729..148432 Replicon chromosome
Accession NZ_CP029727
Organism Citrobacter sp. CRE-46 strain AR_0157

Toxin (Protein)


Gene name ykfI Uniprot ID A0A2Z3WYB7
Locus tag AN232_RS00720 Protein ID WP_031593213.1
Coordinates 147729..148070 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID A0A2Z3WZC0
Locus tag AN232_RS00725 Protein ID WP_032410777.1
Coordinates 148091..148432 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AN232_RS00700 144432..144614 + 183 WP_110496116.1 endoribonuclease -
AN232_RS00705 144661..145296 - 636 WP_110496117.1 HNH endonuclease -
AN232_RS00710 145436..146308 + 873 WP_110496118.1 HNH endonuclease -
AN232_RS00715 146489..147496 - 1008 WP_049017154.1 restriction endonuclease -
AN232_RS00720 147729..148070 - 342 WP_031593213.1 TA system toxin CbtA family protein Toxin
AN232_RS00725 148091..148432 - 342 WP_032410777.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
AN232_RS00730 148443..148985 - 543 WP_100116935.1 DNA repair protein RadC -
AN232_RS00735 148998..149438 - 441 WP_001616563.1 antirestriction protein -
AN232_RS00740 149469..150290 - 822 WP_094986557.1 DUF945 domain-containing protein -
AN232_RS00745 150411..150884 - 474 WP_031593204.1 hypothetical protein -
AN232_RS00750 150956..151408 - 453 WP_031593203.1 hypothetical protein -
AN232_RS00755 151444..152160 - 717 WP_110496119.1 WYL domain-containing protein -
AN232_RS00760 152404..153279 - 876 WP_110496120.1 GTPase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-109)

Antitoxin

Antitoxin CbeA

(13-111)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12736.66 Da        Isoelectric Point: 8.0324

>T106543 WP_031593213.1 NZ_CP029727:c148070-147729 [Citrobacter sp. CRE-46]
MKTLPATTPQAATLCLSPVSVWQMLLARLLEQHYGLTLNDTPFSDETVIQEHIDAGISLADAVNFLVEKYGLVRIDRRGF
DWQEQSPYLRAVDILRARQATGLLKRNRISAAR

Download         Length: 342 bp

>T106543 NZ_CP029727:c148070-147729 [Citrobacter sp. CRE-46]
ATGAAAACTCTACCTGCAACAACTCCGCAGGCGGCGACGCTATGTCTGTCGCCCGTGTCTGTCTGGCAAATGCTGCTTGC
ACGCCTGCTGGAACAACATTATGGCCTGACACTCAACGACACGCCGTTCAGTGATGAAACGGTAATTCAGGAACATATCG
ATGCCGGTATATCTTTGGCCGATGCCGTTAATTTTCTGGTGGAAAAGTACGGGCTGGTTCGTATTGATCGCAGGGGATTC
GACTGGCAAGAACAGTCCCCATATCTGCGGGCAGTCGATATTCTGAGGGCGCGACAGGCAACTGGTTTATTGAAAAGAAA
TCGTATTAGCGCGGCACGGTGA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 12765.40 Da        Isoelectric Point: 7.0269

>AT106543 WP_032410777.1 NZ_CP029727:c148432-148091 [Citrobacter sp. CRE-46]
MKSTDSENDSFLKWGLNRNVTPCFGARLVQEGNRLHYLADRASITGKFSEAECLKLDVAFPHFISQMESMLTTGEMNPRH
AHRVTLYHNGFTCEADTLGSCGYVYIAVYSTHR

Download         Length: 342 bp

>AT106543 NZ_CP029727:c148432-148091 [Citrobacter sp. CRE-46]
ATGAAATCTACTGACTCAGAGAACGATTCATTTCTCAAGTGGGGTCTGAATCGTAATGTAACACCTTGCTTTGGCGCTCG
TCTGGTACAGGAGGGAAACCGGCTGCACTATCTGGCTGACCGGGCCAGTATCACCGGTAAGTTCAGCGAAGCAGAATGTC
TTAAGCTGGATGTGGCATTCCCACATTTTATCAGCCAGATGGAGTCGATGTTGACTACTGGTGAAATGAATCCTCGCCAT
GCCCACCGCGTCACCCTGTACCACAATGGTTTCACCTGCGAAGCCGATACCCTTGGCAGTTGCGGCTACGTATACATCGC
CGTTTACTCCACCCATCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2Z3WYB7

Antitoxin

Source ID Structure
AlphaFold DB A0A2Z3WZC0

References