Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 79441..80177 Replicon plasmid unnamed2
Accession NZ_CP029724
Organism Klebsiella pneumoniae strain AR_0140

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag AM387_RS28075 Protein ID WP_003026803.1
Coordinates 79695..80177 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag AM387_RS28070 Protein ID WP_003026799.1
Coordinates 79441..79707 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM387_RS28025 75503..75865 - 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -
AM387_RS28030 75915..76265 - 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
AM387_RS28035 76623..76892 + 270 WP_004152102.1 hypothetical protein -
AM387_RS28040 76880..77455 + 576 WP_004152103.1 hypothetical protein -
AM387_RS28045 77486..77980 + 495 WP_004152104.1 DNA-binding protein -
AM387_RS28050 78024..78392 + 369 WP_004152105.1 hypothetical protein -
AM387_RS28055 78426..78629 + 204 WP_068814622.1 hemolysin expression modulator Hha -
AM387_RS28060 78678..78935 + 258 WP_004152107.1 hypothetical protein -
AM387_RS28065 79011..79265 + 255 WP_004152108.1 hypothetical protein -
AM387_RS28070 79441..79707 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
AM387_RS28075 79695..80177 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
AM387_RS28080 80385..81731 + 1347 WP_077253535.1 ISNCY family transposase -
AM387_RS28085 81780..82175 + 396 WP_004143398.1 helix-turn-helix domain-containing protein -
AM387_RS28090 82364..83761 + 1398 WP_009653207.1 ISNCY family transposase -
AM387_RS28095 83782..83973 + 192 Protein_84 hypothetical protein -
AM387_RS28100 84001..84279 - 279 WP_004197671.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid catA1 / ere(A) / ARR-3 - 1..178561 178561


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T106538 WP_003026803.1 NZ_CP029724:79695-80177 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T106538 NZ_CP029724:79695-80177 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT106538 WP_003026799.1 NZ_CP029724:79441-79707 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT106538 NZ_CP029724:79441-79707 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References