Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/ParE-RelB |
| Location | 7590..8116 | Replicon | plasmid unnamed4 |
| Accession | NZ_CP029717 | ||
| Organism | Enterobacter cloacae complex sp. strain AR_0154 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | S1EXL1 |
| Locus tag | AM401_RS23355 | Protein ID | WP_000323025.1 |
| Coordinates | 7590..7877 (-) | Length | 96 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | S1F6D3 |
| Locus tag | AM401_RS23360 | Protein ID | WP_000534858.1 |
| Coordinates | 7877..8116 (-) | Length | 80 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| AM401_RS27730 (AM401_23315) | 3160..3336 | - | 177 | WP_001371930.1 | hypothetical protein | - |
| AM401_RS23325 (AM401_23320) | 3847..4791 | + | 945 | WP_000778029.1 | DUF5417 domain-containing protein | - |
| AM401_RS23330 (AM401_23325) | 4887..5489 | + | 603 | WP_012695474.1 | hypothetical protein | - |
| AM401_RS23335 (AM401_23330) | 5549..5899 | + | 351 | WP_000743059.1 | hypothetical protein | - |
| AM401_RS23340 (AM401_23335) | 5946..6149 | + | 204 | WP_001015183.1 | hypothetical protein | - |
| AM401_RS23345 (AM401_23340) | 6431..6751 | + | 321 | WP_000332796.1 | hypothetical protein | - |
| AM401_RS23350 (AM401_23345) | 7364..7489 | - | 126 | WP_229020258.1 | DUF5431 family protein | - |
| AM401_RS23355 (AM401_23350) | 7590..7877 | - | 288 | WP_000323025.1 | type II toxin-antitoxin system mRNA interferase RelE | Toxin |
| AM401_RS23360 (AM401_23355) | 7877..8116 | - | 240 | WP_000534858.1 | type II toxin-antitoxin system antitoxin RelB | Antitoxin |
| AM401_RS28055 | 8141..8245 | + | 105 | Protein_13 | hypothetical protein | - |
| AM401_RS23370 (AM401_23365) | 8379..9302 | - | 924 | WP_000167917.1 | cation diffusion facilitator family transporter | - |
| AM401_RS23375 (AM401_23370) | 9502..10074 | - | 573 | WP_001515348.1 | cytochrome b/b6 domain-containing protein | - |
| AM401_RS23380 (AM401_23375) | 10550..11788 | - | 1239 | WP_000219087.1 | IS110-like element ISEsa2 family transposase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | aac(6')-IIc / ere(A) / qacE / sul1 / sul2 / blaSHV-12 / catA2 / blaTEM-1B / tet(D) / aadA2 / qnrB2 / dfrA19 / aph(3'')-Ib / aph(6)-Id / mcr-9 | - | 1..315949 | 315949 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 96 a.a. Molecular weight: 11225.22 Da Isoelectric Point: 10.1967
>T106514 WP_000323025.1 NZ_CP029717:c7877-7590 [Enterobacter cloacae complex sp.]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL
Download Length: 288 bp
>T106514 NZ_CP029717:c7877-7590 [Enterobacter cloacae complex sp.]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA
Antitoxin
Download Length: 80 a.a. Molecular weight: 9071.48 Da Isoelectric Point: 4.5230
>AT106514 WP_000534858.1 NZ_CP029717:c8116-7877 [Enterobacter cloacae complex sp.]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL
Download Length: 240 bp
>AT106514 NZ_CP029717:c8116-7877 [Enterobacter cloacae complex sp.]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|