Detailed information of TA system TA106512

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 3695778..3696394
• Replicon: chromosome
• Accession: NZ_CP029716
• Organism: Enterobacter cloacae complex sp. strain AR_0154

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: AM401_RS18165
• Protein ID: WP_077266491.1
• Coordinates: 3696023..3696394 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6G4MTK3
• Locus tag: AM401_RS18160
• Protein ID: WP_015569912.1
• Coordinates: 3695778..3696020 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM401_RS18130 (AM401_18125)	3690837..3691637	+	801	WP_003861944.1	DeoR/GlpR family DNA-binding transcription regulator	-
AM401_RS18135 (AM401_18130)	3691756..3692355	+	600	WP_017694048.1	glucose-1-phosphatase	-
AM401_RS18140 (AM401_18135)	3692349..3693230	+	882	WP_003861949.1	virulence factor BrkB family protein	-
AM401_RS18145 (AM401_18140)	3693227..3693664	+	438	WP_015569909.1	D-aminoacyl-tRNA deacylase	-
AM401_RS18150 (AM401_18145)	3693709..3694650	+	942	WP_015569910.1	fatty acid biosynthesis protein FabY	-
AM401_RS18155 (AM401_18150)	3694659..3695579	-	921	WP_110871332.1	alpha/beta hydrolase	-
AM401_RS18160 (AM401_18155)	3695778..3696020	+	243	WP_015569912.1	ribbon-helix-helix domain-containing protein	Antitoxin
AM401_RS18165 (AM401_18160)	3696023..3696394	+	372	WP_077266491.1	type II toxin-antitoxin system VapC family toxin	Toxin
AM401_RS18170 (AM401_18165)	3696434..3697363	-	930	WP_003861956.1	formate dehydrogenase accessory protein FdhE	-
AM401_RS18175 (AM401_18170)	3697360..3697995	-	636	WP_003861958.1	formate dehydrogenase cytochrome b556 subunit	-
AM401_RS18180 (AM401_18175)	3697992..3698894	-	903	WP_014072386.1	formate dehydrogenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-115)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13723.85 Da
• Isoelectric Point: 6.4882

>T106512 WP_077266491.1 NZ_CP029716:3696023-3696394 [Enterobacter cloacae complex sp.]
MEHMAVFDTNILIDLFNNRVEAADAIEHTASHRAISLITWMEVMVGARRHGHEAKTAAVMGAFEIIDVSRDIAERSVLLR
EKHGMKLPDAIILATAQSRKCPLISRNTKDFAGIDEVLTPYQV

Nucleotide

• Length: 372 bp

>T106512 NZ_CP029716:3696023-3696394 [Enterobacter cloacae complex sp.]
ATGGAGCACATGGCAGTGTTTGATACCAATATTCTCATTGATCTCTTCAACAACCGTGTTGAAGCTGCGGATGCGATTGA
GCATACCGCGTCGCATCGTGCCATCAGCTTAATTACCTGGATGGAAGTAATGGTGGGCGCTCGCAGGCATGGTCATGAAG
CGAAAACGGCAGCCGTGATGGGCGCTTTTGAAATTATTGATGTGTCTCGGGATATTGCGGAAAGAAGCGTTTTACTACGT
GAGAAGCATGGAATGAAACTGCCTGATGCTATCATTCTGGCCACAGCGCAGAGCAGAAAATGTCCGTTGATTTCTCGCAA
CACGAAAGATTTTGCGGGTATTGACGAGGTCCTTACGCCTTACCAGGTGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9408.66 Da
• Isoelectric Point: 4.4076

>AT106512 WP_015569912.1 NZ_CP029716:3695778-3696020 [Enterobacter cloacae complex sp.]
VSVLAELDMGRILIDLSDDVIQRLDNLKQLRNQPRAELLREAIELYLDQQSSSVIRNALGLWGDRKEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT106512 NZ_CP029716:3695778-3696020 [Enterobacter cloacae complex sp.]
GTGAGCGTTTTGGCGGAACTTGATATGGGCAGAATACTTATCGATTTGTCTGATGACGTGATTCAACGTTTGGATAATCT
CAAGCAATTGCGAAATCAACCACGTGCTGAACTGCTTCGGGAAGCGATAGAGCTGTATCTCGACCAGCAGAGTTCTTCTG
TTATCCGAAATGCGCTTGGTCTGTGGGGAGACAGAAAAGAGGACGGGCTTGAGTATGAACGCAAGCTGCGTGAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A6G4MTK3