Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 2996941..2997517 Replicon chromosome
Accession NZ_CP029716
Organism Enterobacter cloacae complex sp. strain AR_0154

Toxin (Protein)


Gene name shpA Uniprot ID A0A800YKM1
Locus tag AM401_RS14745 Protein ID WP_015572580.1
Coordinates 2997230..2997517 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A801DSF4
Locus tag AM401_RS14740 Protein ID WP_017694570.1
Coordinates 2996941..2997243 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM401_RS14720 (AM401_14715) 2993237..2993782 + 546 WP_006810254.1 YfaZ family outer membrane protein -
AM401_RS14725 (AM401_14720) 2993959..2995329 - 1371 WP_110871120.1 NAD-dependent succinate-semialdehyde dehydrogenase -
AM401_RS14730 (AM401_14725) 2995483..2996235 + 753 WP_033486709.1 AraC family transcriptional regulator -
AM401_RS14735 (AM401_14730) 2996274..2996912 + 639 WP_110871122.1 LysE family translocator -
AM401_RS14740 (AM401_14735) 2996941..2997243 - 303 WP_017694570.1 BrnA antitoxin family protein Antitoxin
AM401_RS14745 (AM401_14740) 2997230..2997517 - 288 WP_015572580.1 BrnT family toxin Toxin
AM401_RS14750 (AM401_14745) 2997687..2998958 + 1272 WP_110871124.1 DUF445 domain-containing protein -
AM401_RS14755 (AM401_14750) 2998955..3000031 - 1077 WP_015572578.1 DUF2955 domain-containing protein -
AM401_RS14760 (AM401_14755) 3000021..3001088 - 1068 WP_108394134.1 HlyD family secretion protein -
AM401_RS14765 (AM401_14760) 3001085..3001555 - 471 WP_023295868.1 MarR family transcriptional regulator -
AM401_RS14770 (AM401_14765) 3001705..3002424 - 720 WP_045330246.1 winged helix-turn-helix domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(32-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11296.81 Da        Isoelectric Point: 7.3587

>T106511 WP_015572580.1 NZ_CP029716:c2997517-2997230 [Enterobacter cloacae complex sp.]
MPIEYEWDSNKAKSNLQKHAIRFEDAVLVFDDPCHLSVQDRYENGEFRWQTIGLVQGVIVILVAHTVRFESGDEIIRIIS
ARKADRKERRRYEHR

Download         Length: 288 bp

>T106511 NZ_CP029716:c2997517-2997230 [Enterobacter cloacae complex sp.]
ATGCCTATAGAGTATGAATGGGATAGCAATAAGGCGAAAAGCAATCTGCAAAAACACGCCATTCGTTTTGAAGATGCAGT
TCTGGTTTTTGACGATCCCTGCCATTTGTCTGTTCAGGATCGTTATGAAAACGGGGAGTTTCGCTGGCAAACCATTGGTC
TTGTACAGGGTGTTATTGTCATTCTGGTCGCGCACACGGTACGTTTTGAAAGCGGGGATGAAATCATACGTATCATCAGT
GCGCGTAAAGCGGATCGTAAAGAGAGGAGGCGTTATGAGCATCGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11482.13 Da        Isoelectric Point: 10.4094

>AT106511 WP_017694570.1 NZ_CP029716:c2997243-2996941 [Enterobacter cloacae complex sp.]
MSIVKHKRGSAPEVSARREAELKALADKPDREIDYSDIPATEDDRWAGAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT106511 NZ_CP029716:c2997243-2996941 [Enterobacter cloacae complex sp.]
ATGAGCATCGTTAAACATAAACGCGGTAGTGCACCGGAAGTGAGTGCCCGCCGGGAGGCTGAACTCAAAGCGCTGGCGGA
TAAACCCGACAGGGAAATTGATTACAGCGATATACCGGCCACGGAGGATGATCGCTGGGCCGGTGCCGTGCGGGGAAAAT
TCTATCGACCGCTCAAAACTCAGGCATCAGTACGCATTGACGCCGACGTGATGGAGTGGCTTAAACGGCCAGGGAAAGGT
TACCAGACGCGGCTTAATGCCATTTTGCGCGAGGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References