Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2527954..2528574 Replicon chromosome
Accession NZ_CP029716
Organism Enterobacter cloacae complex sp. strain AR_0154

Toxin (Protein)


Gene name Hha Uniprot ID A0A837FFM2
Locus tag AM401_RS12525 Protein ID WP_015571250.1
Coordinates 2528356..2528574 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID F5RUW7
Locus tag AM401_RS12520 Protein ID WP_006809850.1
Coordinates 2527954..2528328 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM401_RS12510 (AM401_12505) 2523081..2524274 + 1194 WP_017694395.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
AM401_RS12515 (AM401_12510) 2524297..2527443 + 3147 WP_015571248.1 multidrug efflux RND transporter permease subunit AcrB -
AM401_RS12520 (AM401_12515) 2527954..2528328 + 375 WP_006809850.1 Hha toxicity modulator TomB Antitoxin
AM401_RS12525 (AM401_12520) 2528356..2528574 + 219 WP_015571250.1 HHA domain-containing protein Toxin
AM401_RS12530 (AM401_12525) 2528783..2529334 + 552 WP_110870982.1 maltose O-acetyltransferase -
AM401_RS12535 (AM401_12530) 2529451..2529918 + 468 WP_023296041.1 YlaC family protein -
AM401_RS12540 (AM401_12535) 2529890..2531350 - 1461 WP_110870983.1 PLP-dependent aminotransferase family protein -
AM401_RS12545 (AM401_12540) 2531452..2532162 + 711 WP_110870984.1 GNAT family protein -
AM401_RS12550 (AM401_12545) 2532159..2532299 - 141 WP_003859006.1 type B 50S ribosomal protein L36 -
AM401_RS12555 (AM401_12550) 2532302..2532562 - 261 WP_015571255.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8583.95 Da        Isoelectric Point: 8.9008

>T106510 WP_015571250.1 NZ_CP029716:2528356-2528574 [Enterobacter cloacae complex sp.]
MSDKPLTKVDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFVR

Download         Length: 219 bp

>T106510 NZ_CP029716:2528356-2528574 [Enterobacter cloacae complex sp.]
ATGTCTGATAAACCATTAACAAAAGTCGATTATTTGATGCGCCTGCGACGCTGCCAGTCAATTGACACCCTTGAACGAGT
TATTGAGAAAAATAAATACGAGCTGTCTGATAATGAACTGGCGGTATTTTATTCAGCTGCCGACCATCGTCTTGCTGAGC
TGACCATGAATAAACTGTATGACAAGATCCCCTCTTCGGTGTGGAAATTTGTTCGTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14497.28 Da        Isoelectric Point: 4.8989

>AT106510 WP_006809850.1 NZ_CP029716:2527954-2528328 [Enterobacter cloacae complex sp.]
MDEYSPKRHDIAQLKFLCESLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFTNVSRANPVSLSC

Download         Length: 375 bp

>AT106510 NZ_CP029716:2527954-2528328 [Enterobacter cloacae complex sp.]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGAAGAAAGTAACCATGGCTGGGTGAACGATCCAACGTCTGCTATCAATTTACAGCTTAACGAGCTGATCGAGC
ATATCGCCACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGTTGATAGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTACGGCATTAACGCACAGGATTTGCAGAAATGGCGTAAATCAGGAAACCGTCT
ATTTCGCTGCTTCACTAATGTCAGCAGAGCTAATCCGGTTAGTCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A837FFM2

Antitoxin

Source ID Structure
AlphaFold DB A0A837FGN8

References