Detailed information of TA system TA106476

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 4180518..4181104
• Replicon: chromosome
• Accession: NZ_CP029707
• Organism: Pseudomonas aeruginosa strain K34-7

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: DCS61_RS20220
• Protein ID: WP_003120987.1
• Coordinates: 4180805..4181104 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: DCS61_RS20215
• Protein ID: WP_110594072.1
• Coordinates: 4180518..4180808 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DCS61_RS20200	4176100..4177989	+	1890	WP_110594070.1	hypothetical protein	-
DCS61_RS20205	4177986..4179962	+	1977	WP_110594071.1	DEAD/DEAH box helicase	-
DCS61_RS20210	4180103..4180447	+	345	WP_003448665.1	hypothetical protein	-
DCS61_RS20215	4180518..4180808	-	291	WP_110594072.1	putative addiction module antidote protein	Antitoxin
DCS61_RS20220	4180805..4181104	-	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DCS61_RS20225	4181306..4182430	+	1125	WP_110594073.1	TcpQ domain-containing protein	-
DCS61_RS20230	4182430..4184139	+	1710	WP_003123331.1	PilN family type IVB pilus formation outer membrane protein	-
DCS61_RS20235	4184143..4185468	+	1326	WP_003120992.1	type 4b pilus protein PilO2	-
DCS61_RS20240	4185458..4185991	+	534	WP_110594074.1	type IV pilus biogenesis protein PilP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	4153870..4238811	84941

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T106476 WP_003120987.1 NZ_CP029707:c4181104-4180805 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T106476 NZ_CP029707:c4181104-4180805 [Pseudomonas aeruginosa]
ATGGTTGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.96 Da
• Isoelectric Point: 5.0774

>AT106476 WP_110594072.1 NZ_CP029707:c4180808-4180518 [Pseudomonas aeruginosa]
MTEKIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT106476 NZ_CP029707:c4180808-4180518 [Pseudomonas aeruginosa]
ATGACCGAGAAAATTTATGATTATGACCCCGCGCAAGCGCTGGACAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
TTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCCCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAGTCGTTTAGTGAGCGCGGGAACCCTACATTGAAAACCATGCTGGCA
GTAATGCGAGCTCTGGGAGTTGATCTGACCGCCCGCCCGCATGCTCAGTAA

Structures

Toxin

• AlphaFold DB: G8CP73