Detailed information of TA system TA106468

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RHH
• Location: 5641243..5641756
• Replicon: chromosome
• Accession: NZ_CP029693
• Organism: Pseudomonas putida strain JBC17

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: DKY63_RS26410
• Protein ID: WP_110966812.1
• Coordinates: 5641243..5641527 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: DKY63_RS26415
• Protein ID: WP_110966813.1
• Coordinates: 5641517..5641756 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DKY63_RS26390	5637247..5638173	+	927	WP_110966808.1	hydroxymethylglutaryl-CoA lyase	-
DKY63_RS26395	5638356..5639687	+	1332	WP_110966809.1	MFS transporter	-
DKY63_RS26400	5639766..5640254	-	489	WP_110966810.1	DMT family transporter	-
DKY63_RS26405	5640332..5641234	+	903	WP_110966811.1	LysR family transcriptional regulator	-
DKY63_RS26410	5641243..5641527	-	285	WP_110966812.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DKY63_RS26415	5641517..5641756	-	240	WP_110966813.1	ribbon-helix-helix protein, CopG family	Antitoxin
DKY63_RS26420	5641910..5642548	+	639	WP_110966814.1	LysE family translocator	-
DKY63_RS26425	5642636..5643982	+	1347	WP_110966815.1	sensor domain-containing diguanylate cyclase	-
DKY63_RS26435	5644337..5644840	+	504	WP_110966817.1	DUF4142 domain-containing protein	-
DKY63_RS26440	5644886..5645296	-	411	WP_110966818.1	low affinity iron permease family protein	-
DKY63_RS26445	5645427..5646197	+	771	WP_110966819.1	trans-aconitate 2-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-89)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-41)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10978.70 Da
• Isoelectric Point: 10.3378

>T106468 WP_110966812.1 NZ_CP029693:c5641527-5641243 [Pseudomonas putida]
MRVEWLRTALKNLDDEAAYIAKENPKAAADFVQAVFASVDHLAHFPATGHEGRLPGTREWVMTDRPYLIPYRVRKDRLQV
LRIFHTRRLPPALW

Nucleotide

• Length: 285 bp

>T106468 NZ_CP029693:c5641527-5641243 [Pseudomonas putida]
ATGCGCGTTGAGTGGCTACGTACGGCGTTGAAAAATCTTGATGACGAAGCCGCATACATCGCAAAAGAGAATCCAAAGGC
TGCCGCAGATTTCGTCCAGGCTGTATTCGCCAGCGTGGACCATCTTGCTCACTTTCCAGCGACCGGCCATGAAGGGCGTT
TACCCGGCACCCGGGAATGGGTCATGACCGATCGCCCCTATCTGATTCCCTATCGCGTTCGTAAAGATCGCTTACAGGTT
CTTCGAATCTTTCACACACGTCGCCTTCCACCTGCTCTTTGGTAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8716.84 Da
• Isoelectric Point: 4.5881

>AT106468 WP_110966813.1 NZ_CP029693:c5641756-5641517 [Pseudomonas putida]
MSVMSLRVPDEIADTLASLSKATGRSKSFLAVDALREYLAREAWQIEEIQKALKEADAGDFATTDEVKAIADKWTTNAR

Nucleotide

• Length: 240 bp

>AT106468 NZ_CP029693:c5641756-5641517 [Pseudomonas putida]
ATGTCAGTCATGTCCCTGCGCGTACCCGATGAAATCGCCGATACCCTCGCCAGCCTTTCCAAGGCAACTGGCCGCAGCAA
ATCATTTTTGGCCGTGGATGCGTTACGGGAGTACCTGGCCCGGGAAGCTTGGCAAATAGAAGAGATTCAGAAAGCACTGA
AAGAAGCCGATGCCGGTGACTTCGCAACAACAGATGAAGTGAAAGCTATCGCTGACAAGTGGACAACCAATGCGCGTTGA