Detailed information of TA system TA106454

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3141250..3141875
• Replicon: chromosome
• Accession: NZ_CP029692
• Organism: Escherichia coli strain SD134209

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: DL800_RS18780
• Protein ID: WP_000911330.1
• Coordinates: 3141250..3141648 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: DL800_RS18785
• Protein ID: WP_000450524.1
• Coordinates: 3141648..3141875 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DL800_RS18760	3137128..3137328	+	201	WP_000383836.1	YpfN family protein	-
DL800_RS18765	3137438..3138136	-	699	WP_000679812.1	esterase	-
DL800_RS18770	3138210..3140225	-	2016	WP_000829332.1	tRNA cytosine(34) acetyltransferase TmcA	-
DL800_RS18775	3140240..3141103	-	864	WP_001267508.1	neutral zinc metallopeptidase	-
DL800_RS18780	3141250..3141648	-	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
DL800_RS18785	3141648..3141875	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
DL800_RS18790	3142029..3142742	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
DL800_RS18795	3142955..3143989	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
DL800_RS18800	3144006..3144884	-	879	WP_001311023.1	4-hydroxy-tetrahydrodipicolinate synthase	-
DL800_RS18805	3145030..3145614	+	585	WP_000176194.1	glycine cleavage system transcriptional repressor	-
DL800_RS18810	3145614..3146084	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T106454 WP_000911330.1 NZ_CP029692:c3141648-3141250 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T106454 NZ_CP029692:c3141648-3141250 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT106454 WP_000450524.1 NZ_CP029692:c3141875-3141648 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT106454 NZ_CP029692:c3141875-3141648 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33