Detailed information of TA system TA106442

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 1380858..1381329
• Replicon: chromosome
• Accession: NZ_CP029692
• Organism: Escherichia coli strain SD134209

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0D7C2L1
• Locus tag: DL800_RS08875
• Protein ID: WP_001303511.1
• Coordinates: 1380858..1381136 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XAD5
• Locus tag: DL800_RS08880
• Protein ID: WP_001302048.1
• Coordinates: 1381138..1381329 (-)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DL800_RS08835	1376165..1376434	-	270	WP_000003742.1	excisionase	-
DL800_RS08840	1376496..1378961	-	2466	WP_000048442.1	exonuclease	-
DL800_RS08845	1379054..1379245	-	192	WP_001090200.1	DUF1482 family protein	-
DL800_RS08850	1379242..1379430	-	189	WP_000449192.1	cell division inhibition protein DicB	-
DL800_RS08860	1379914..1380132	-	219	WP_001171930.1	hypothetical protein	-
DL800_RS08865	1380173..1380562	-	390	WP_001447517.1	hypothetical protein	-
DL800_RS08875	1380858..1381136	-	279	WP_001303511.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DL800_RS08880	1381138..1381329	-	192	WP_001302048.1	hypothetical protein	Antitoxin
DL800_RS08885	1381350..1381721	-	372	WP_001169687.1	hypothetical protein	-
DL800_RS08890	1381819..1382121	+	303	WP_001444689.1	transcriptional regulator	-
DL800_RS08895	1382118..1382261	+	144	Protein_1320	Rha family transcriptional regulator	-
DL800_RS08900	1382318..1383531	+	1214	WP_162829202.1	IS3 family transposase	-
DL800_RS08905	1383575..1383856	+	282	Protein_1322	toxin YdaT domain-containing protein	-
DL800_RS08910	1383879..1384841	+	963	WP_000095671.1	helix-turn-helix domain-containing protein	-
DL800_RS08915	1384882..1385304	+	423	WP_001151120.1	DUF977 family protein	-
DL800_RS08920	1385301..1385555	+	255	WP_000004322.1	hypothetical protein	-
DL800_RS08925	1385548..1385859	+	312	WP_001002672.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleC	1367909..1425729	57820

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10661.25 Da
• Isoelectric Point: 5.5647

>T106442 WP_001303511.1 NZ_CP029692:c1381136-1380858 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Nucleotide

• Length: 279 bp

>T106442 NZ_CP029692:c1381136-1380858 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7463.47 Da
• Isoelectric Point: 8.6828

>AT106442 WP_001302048.1 NZ_CP029692:c1381329-1381138 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Nucleotide

• Length: 192 bp

>AT106442 NZ_CP029692:c1381329-1381138 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• PDB: 5CZF
• PDB: 5CW7
• PDB: 5CZE

Antitoxin

• PDB: 5CZF
• PDB: 3ZBE
• PDB: 5CW7
• AlphaFold DB: A0A829CTP0