106428

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2291313..2291938 Replicon chromosome
Accession NZ_CP029689
Organism Klebsiella pneumoniae strain 160111

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag DL890_RS11620 Protein ID WP_002882817.1
Coordinates 2291555..2291938 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag DL890_RS11615 Protein ID WP_004150355.1
Coordinates 2291313..2291555 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DL890_RS11590 2287304..2287903 + 600 WP_004151865.1 glucose-1-phosphatase -
DL890_RS11595 2287897..2288757 + 861 WP_002882807.1 virulence factor BrkB family protein -
DL890_RS11600 2288754..2289191 + 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
DL890_RS11605 2289236..2290177 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
DL890_RS11610 2290191..2291108 - 918 WP_002882812.1 alpha/beta hydrolase -
DL890_RS11615 2291313..2291555 + 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
DL890_RS11620 2291555..2291938 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
DL890_RS11625 2292112..2293041 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
DL890_RS11630 2293038..2293673 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
DL890_RS11635 2293670..2294572 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T106428 WP_002882817.1 NZ_CP029689:2291555-2291938 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T106428 NZ_CP029689:2291555-2291938 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT106428 WP_004150355.1 NZ_CP029689:2291313-2291555 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT106428 NZ_CP029689:2291313-2291555 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References