Detailed information of TA system TA106415

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 97391..97992
• Replicon: plasmid pLKJULY7989
• Accession: NZ_CP029688
• Organism: Escherichia coli strain E706

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: DMI84_RS25525
• Protein ID: WP_001216039.1
• Coordinates: 97391..97771 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: DMI84_RS25530
• Protein ID: WP_001190711.1
• Coordinates: 97771..97992 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DMI84_RS25485	92517..93221	+	705	WP_001067858.1	IS6-like element IS26 family transposase	-
DMI84_RS25490	93274..93651	+	378	Protein_96	recombinase family protein	-
DMI84_RS25495	93834..94694	+	861	WP_000027057.1	class A broad-spectrum beta-lactamase TEM-1	-
DMI84_RS25505	94914..95611	-	698	Protein_98	IS1 family transposase	-
DMI84_RS25515	95690..97156	+	1467	WP_074139093.1	PAS domain-containing methyl-accepting chemotaxis protein	-
DMI84_RS25525	97391..97771	-	381	WP_001216039.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
DMI84_RS25530	97771..97992	-	222	WP_001190711.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
DMI84_RS25540	98257..98954	+	698	Protein_102	IS1 family transposase	-
DMI84_RS25545	98971..99297	-	327	Protein_103	hypothetical protein	-
DMI84_RS25550	99370..100341	-	972	WP_118014891.1	hypothetical protein	-
DMI84_RS25555	100448..101152	+	705	WP_001067858.1	IS6-like element IS26 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	aadA2 / dfrA12 / floR / sul2 / blaTEM-1B / aph(3'')-Ib / aph(6)-Id / mph(A)	-	1..151879	151879
	-	inside	IScluster/Tn	blaTEM-1B / sul2 / aph(3'')-Ib / aph(6)-Id / mph(A)	-	90669..126220	35551

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (2-123)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13558.27 Da
• Isoelectric Point: 5.1514

>T106415 WP_001216039.1 NZ_CP029688:c97771-97391 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRAALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T106415 NZ_CP029688:c97771-97391 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTGATAGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCTGGCATGTC
AGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTGCCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCCGCGGAGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8134.08 Da
• Isoelectric Point: 4.5843

>AT106415 WP_001190711.1 NZ_CP029688:c97992-97771 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVDR

Nucleotide

• Length: 222 bp

>AT106415 NZ_CP029688:c97992-97771 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCTCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTGACCGATAA