Detailed information of TA system TA106413

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataT-KacA/DUF1778(antitoxin)
• Location: 31866..32664
• Replicon: plasmid pLKJULY7989
• Accession: NZ_CP029688
• Organism: Escherichia coli strain E706

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: DMI84_RS25080
• Protein ID: WP_072662620.1
• Coordinates: 31866..32387 (-)
• Length: 174 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: A0A141BRA2
• Locus tag: DMI84_RS25085
• Protein ID: WP_001711191.1
• Coordinates: 32395..32664 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DMI84_RS25045	27881..28138	+	258	Protein_17	IS3 family transposase	-
DMI84_RS25050	28151..29298	-	1148	Protein_18	IS30-like element IS30 family transposase	-
DMI84_RS25055	29363..29959	+	597	Protein_19	IS3 family transposase	-
DMI84_RS25065	30241..31317	+	1077	WP_038989268.1	IS110-like element ISEc21 family transposase	-
DMI84_RS25070	31334..31642	+	309	Protein_21	DDE-type integrase/transposase/recombinase	-
DMI84_RS26145	31657..31800	-	144	WP_162618456.1	hypothetical protein	-
DMI84_RS25080	31866..32387	-	522	WP_072662620.1	GNAT family N-acetyltransferase	Toxin
DMI84_RS25085	32395..32664	-	270	WP_001711191.1	DUF1778 domain-containing protein	Antitoxin
DMI84_RS25090	32984..33649	+	666	WP_023135658.1	AAA family ATPase	-
DMI84_RS25095	33655..34008	+	354	WP_161953766.1	hypothetical protein	-
DMI84_RS25100	34061..34828	-	768	WP_001711193.1	hypothetical protein	-
DMI84_RS25105	35018..36246	+	1229	WP_152951998.1	IS3 family transposase	-
DMI84_RS25110	36258..36353	-	96	Protein_29	PerC family transcriptional regulator	-
DMI84_RS25115	36443..37177	+	735	WP_000578338.1	helix-turn-helix domain-containing protein	-
DMI84_RS25120	37332..37526	-	195	Protein_31	iron-containing alcohol dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	aadA2 / dfrA12 / floR / sul2 / blaTEM-1B / aph(3'')-Ib / aph(6)-Id / mph(A)	-	1..151879	151879
	-	inside	IScluster/Tn	aadA2 / dfrA12 / floR / sul2	-	27881..62977	35096

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (48-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 174 a.a.
• Molecular weight: 19359.18 Da
• Isoelectric Point: 8.2462

>T106413 WP_072662620.1 NZ_CP029688:c32387-31866 [Escherichia coli]
VSNTTIEIFSGEKDYDLNGFDCGEESLNAFLTNHLKRQHEGKILRAYVLCTKEEIPKVLGYYTLSGSCFEKETLPSKSQQ
KKVPYRNVPSITLGRLALDKSLQGQGLGSMLVTHAMRVVYNASLAVGIHGLFVEALNDKARAFYKSLGFIQLVGNNDRSL
FYPTKSIEKLFEE

Nucleotide

• Length: 522 bp

>T106413 NZ_CP029688:c32387-31866 [Escherichia coli]
GTGAGCAATACGACGATCGAGATTTTCTCTGGAGAGAAAGATTATGATCTAAACGGTTTTGATTGCGGCGAAGAGTCACT
AAACGCATTTTTGACCAACCACTTAAAAAGACAGCATGAAGGAAAAATTCTTCGCGCTTATGTGCTTTGCACTAAAGAAG
AAATACCAAAGGTATTAGGTTATTACACTTTGTCAGGCAGCTGCTTTGAGAAAGAAACCTTACCTTCAAAGAGTCAGCAA
AAGAAAGTGCCTTATCGGAATGTCCCAAGTATCACTTTAGGCAGACTGGCTTTGGACAAATCTCTTCAAGGTCAGGGGCT
TGGCTCAATGCTTGTAACTCACGCAATGCGTGTTGTGTACAATGCATCTCTTGCGGTAGGCATTCATGGGCTTTTCGTTG
AGGCGTTGAACGATAAAGCCAGGGCGTTTTATAAAAGTTTGGGCTTTATCCAGCTGGTTGGTAACAACGATCGTTCTTTG
TTCTATCCAACAAAATCTATCGAAAAATTGTTCGAAGAATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10054.53 Da
• Isoelectric Point: 8.7353

>AT106413 WP_001711191.1 NZ_CP029688:c32664-32395 [Escherichia coli]
MSALKKQRIDLRLTDDDKSIIEEAAAMSNQSITQFMVSSASERAVKVIEQHRRLVLSEESWNLVMDAISNPPAPNDKLKR
AANRLKSME

Nucleotide

• Length: 270 bp

>AT106413 NZ_CP029688:c32664-32395 [Escherichia coli]
ATGTCCGCACTGAAAAAGCAGCGCATCGATTTGAGATTAACCGATGACGATAAAAGCATCATCGAGGAAGCTGCCGCAAT
GTCTAACCAGAGCATTACTCAGTTTATGGTTAGCAGTGCATCCGAACGTGCCGTGAAGGTGATAGAGCAACACCGTAGAC
TGGTTCTAAGTGAAGAGTCCTGGAATCTGGTTATGGATGCTATAAGCAATCCTCCGGCACCGAACGACAAGCTGAAACGA
GCTGCCAATCGTCTGAAAAGCATGGAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A141BRA2