Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 3765..4290 Replicon plasmid pFORC89
Accession NZ_CP029683
Organism Salmonella sp. FORC89

Toxin (Protein)


Gene name ccdB Uniprot ID I3W3D5
Locus tag FORC89_RS22770 Protein ID WP_001159863.1
Coordinates 3985..4290 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID M7S5D0
Locus tag FORC89_RS22765 Protein ID WP_000813641.1
Coordinates 3765..3983 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FORC89_RS22745 (FORC89_p002) 306..1295 - 990 WP_000461382.1 RepB family plasmid replication initiator protein -
FORC89_RS22750 1789..2084 - 296 Protein_1 cytoplasmic protein -
FORC89_RS22755 2096..2524 + 429 Protein_2 hypothetical protein -
FORC89_RS22760 (FORC89_p005) 2568..3089 - 522 WP_077681952.1 hypothetical protein -
FORC89_RS22765 (FORC89_p008) 3765..3983 + 219 WP_000813641.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
FORC89_RS22770 (FORC89_p009) 3985..4290 + 306 WP_001159863.1 type II toxin-antitoxin system toxin CcdB Toxin
FORC89_RS22775 (FORC89_p010) 4292..4582 + 291 WP_001266176.1 hypothetical protein -
FORC89_RS22780 (FORC89_p011) 4579..5100 + 522 WP_000198608.1 hypothetical protein -
FORC89_RS22785 (FORC89_p012) 5135..5917 + 783 WP_000082169.1 site-specific integrase -
FORC89_RS22790 (FORC89_p013) 5926..6639 + 714 WP_000545756.1 EAL domain-containing protein -
FORC89_RS22795 (FORC89_p014) 6664..7152 + 489 WP_001075507.1 CaiF/GrlA family transcriptional regulator -
FORC89_RS22800 (FORC89_p015) 7146..7631 + 486 WP_000905606.1 membrane protein -
FORC89_RS22805 7908..8195 - 288 WP_071530243.1 hypothetical protein -
FORC89_RS22810 8351..8911 + 561 WP_000900095.1 inverse autotransporter beta domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid catA2 / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B fdeC / spvC / spvB / rck / pefD / pefC / pefA / pefB 1..86805 86805


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11587.48 Da        Isoelectric Point: 6.9787

>T106372 WP_001159863.1 NZ_CP029683:3985-4290 [Salmonella sp. FORC89]
MQFKVYTCKRESRYRLFVDVQSDIIDTPGRRMAVPLVSARLLSEKVPRDLYPVMHIGDEPYRLLTTDMTSVPATVIGEEV
ADLSLRENDIKNAINLMFRGI

Download         Length: 306 bp

>T106372 NZ_CP029683:3985-4290 [Salmonella sp. FORC89]
ATGCAGTTTAAGGTTTACACCTGTAAAAGGGAGAGTCGCTACCGGCTGTTTGTGGACGTGCAGAGCGACATCATTGACAC
CCCCGGACGGCGGATGGCTGTCCCGCTGGTCAGCGCCCGTCTGCTGTCGGAAAAGGTTCCCCGCGATCTTTACCCGGTGA
TGCATATCGGGGATGAGCCTTACCGCCTGCTGACCACGGATATGACCAGTGTGCCGGCCACCGTCATCGGGGAAGAGGTG
GCCGATCTCAGCCTCCGGGAGAACGATATCAAAAACGCCATTAACCTGATGTTCCGGGGGATCTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8314.16 Da        Isoelectric Point: 4.5203

>AT106372 WP_000813641.1 NZ_CP029683:3765-3983 [Salmonella sp. FORC89]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQEENREGMAEVASFIEANGSFADDNRNW

Download         Length: 219 bp

>AT106372 NZ_CP029683:3765-3983 [Salmonella sp. FORC89]
ATGAAGCAGCGAATTACAGTGACAGTGGACAGCGACAGCTATCAGCTGCTCAAGGCATACGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGCCGTCTGCGAGCCGAACGCTGGCAGGAAGAAAATCGGGAAGGTATGGCTG
AGGTGGCCAGCTTTATAGAGGCTAACGGATCGTTTGCTGACGACAACAGGAACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB I3W3D5

Antitoxin

Source ID Structure
AlphaFold DB A0A656ICA6

References