Detailed information of TA system TA106278

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2197007..2197536
• Replicon: chromosome
• Accession: NZ_CP029675
• Organism: Staphylococcus aureus strain AR_0219

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: CSB75_RS11665
• Protein ID: WP_000621175.1
• Coordinates: 2197007..2197369 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: CSB75_RS11670
• Protein ID: WP_000948331.1
• Coordinates: 2197366..2197536 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSB75_RS11640	2193986..2194756	-	771	WP_001041106.1	RNA polymerase sigma factor SigB	-
CSB75_RS11645	2194731..2195210	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
CSB75_RS11650	2195212..2195538	-	327	WP_001052491.1	anti-sigma factor antagonist	-
CSB75_RS11655	2195657..2196658	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
CSB75_RS11665	2197007..2197369	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CSB75_RS11670	2197366..2197536	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
CSB75_RS11675	2197621..2198769	-	1149	WP_001281154.1	alanine racemase	-
CSB75_RS11680	2198835..2199194	-	360	WP_000581197.1	holo-ACP synthase	-
CSB75_RS11685	2199198..2199689	-	492	WP_001205912.1	PH domain-containing protein	-
CSB75_RS11690	2199676..2201259	-	1584	WP_001294626.1	PH domain-containing protein	-
CSB75_RS11695	2201252..2201731	-	480	WP_001287087.1	hypothetical protein	-
CSB75_RS11700	2201939..2202499	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T106278 WP_000621175.1 NZ_CP029675:c2197369-2197007 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T106278 NZ_CP029675:c2197369-2197007 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT106278 WP_000948331.1 NZ_CP029675:c2197536-2197366 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT106278 NZ_CP029675:c2197536-2197366 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1