Detailed information of TA system TA106250

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2109115..2109644
• Replicon: chromosome
• Accession: NZ_CP029671
• Organism: Staphylococcus aureus strain AR_0222

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: CSB78_RS10785
• Protein ID: WP_000621175.1
• Coordinates: 2109282..2109644 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: CSB78_RS10780
• Protein ID: WP_000948331.1
• Coordinates: 2109115..2109285 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSB78_RS10750	2104152..2104712	+	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-
CSB78_RS10755	2104920..2105399	+	480	WP_001287087.1	hypothetical protein	-
CSB78_RS10760	2105392..2106975	+	1584	WP_001294626.1	PH domain-containing protein	-
CSB78_RS10765	2106962..2107453	+	492	WP_001205912.1	PH domain-containing protein	-
CSB78_RS10770	2107457..2107816	+	360	WP_000581197.1	holo-ACP synthase	-
CSB78_RS10775	2107882..2109030	+	1149	WP_001281154.1	alanine racemase	-
CSB78_RS10780	2109115..2109285	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
CSB78_RS10785	2109282..2109644	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CSB78_RS10795	2109994..2110995	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
CSB78_RS10800	2111114..2111440	+	327	WP_001052491.1	anti-sigma factor antagonist	-
CSB78_RS10805	2111442..2111921	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
CSB78_RS10810	2111896..2112666	+	771	WP_001041111.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T106250 WP_000621175.1 NZ_CP029671:2109282-2109644 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T106250 NZ_CP029671:2109282-2109644 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT106250 WP_000948331.1 NZ_CP029671:2109115-2109285 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT106250 NZ_CP029671:2109115-2109285 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1