Detailed information of TA system TA106219

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 56207..56736
• Replicon: chromosome
• Accession: NZ_CP029669
• Organism: Staphylococcus aureus strain AR_0223

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: CSB79_RS00340
• Protein ID: WP_000621175.1
• Coordinates: 56374..56736 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: CSB79_RS00335
• Protein ID: WP_000948331.1
• Coordinates: 56207..56377 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSB79_RS00305	51244..51804	+	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-
CSB79_RS00310	52012..52491	+	480	WP_001287088.1	hypothetical protein	-
CSB79_RS00315	52484..54067	+	1584	WP_001294626.1	PH domain-containing protein	-
CSB79_RS00320	54054..54545	+	492	WP_110185341.1	PH domain-containing protein	-
CSB79_RS00325	54549..54908	+	360	WP_000581200.1	holo-ACP synthase	-
CSB79_RS00330	54974..56122	+	1149	WP_001281145.1	alanine racemase	-
CSB79_RS00335	56207..56377	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
CSB79_RS00340	56374..56736	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CSB79_RS00350	57086..58087	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
CSB79_RS00355	58206..58532	+	327	WP_001052491.1	anti-sigma factor antagonist	-
CSB79_RS00360	58534..59013	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
CSB79_RS00365	58988..59758	+	771	WP_001041103.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T106219 WP_000621175.1 NZ_CP029669:56374-56736 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T106219 NZ_CP029669:56374-56736 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT106219 WP_000948331.1 NZ_CP029669:56207-56377 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT106219 NZ_CP029669:56207-56377 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1