Detailed information of TA system TA106086

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2366197..2366726
• Replicon: chromosome
• Accession: NZ_CP029652
• Organism: Staphylococcus aureus strain AR_0471

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: CSC56_RS12240
• Protein ID: WP_000621175.1
• Coordinates: 2366364..2366726 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: CSC56_RS12235
• Protein ID: WP_000948331.1
• Coordinates: 2366197..2366367 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSC56_RS12205	2361234..2361794	+	561	WP_001092406.1	K(+)-transporting ATPase subunit C	-
CSC56_RS12210	2362002..2362481	+	480	WP_001287077.1	hypothetical protein	-
CSC56_RS12215	2362474..2364057	+	1584	WP_001294637.1	PH domain-containing protein	-
CSC56_RS12220	2364044..2364535	+	492	WP_001205907.1	PH domain-containing protein	-
CSC56_RS12225	2364539..2364898	+	360	WP_000581199.1	holo-ACP synthase	-
CSC56_RS12230	2364964..2366112	+	1149	WP_001281140.1	alanine racemase	-
CSC56_RS12235	2366197..2366367	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
CSC56_RS12240	2366364..2366726	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CSC56_RS12250	2367076..2368077	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
CSC56_RS12255	2368196..2368522	+	327	WP_001052491.1	anti-sigma factor antagonist	-
CSC56_RS12260	2368524..2369003	+	480	WP_001190825.1	anti-sigma B factor RsbW	-
CSC56_RS12265	2368978..2369748	+	771	WP_001041107.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T106086 WP_000621175.1 NZ_CP029652:2366364-2366726 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T106086 NZ_CP029652:2366364-2366726 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAGCAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT106086 WP_000948331.1 NZ_CP029652:2366197-2366367 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT106086 NZ_CP029652:2366197-2366367 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1