Detailed information of TA system TA106074

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2470712..2471241
• Replicon: chromosome
• Accession: NZ_CP029649
• Organism: Staphylococcus aureus strain AR_0472

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: CSC57_RS12585
• Protein ID: WP_000621175.1
• Coordinates: 2470879..2471241 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: CSC57_RS12580
• Protein ID: WP_000948331.1
• Coordinates: 2470712..2470882 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSC57_RS12550	2465748..2466308	+	561	WP_001092402.1	K(+)-transporting ATPase subunit C	-
CSC57_RS12555	2466517..2466996	+	480	WP_001287090.1	hypothetical protein	-
CSC57_RS12560	2466989..2468572	+	1584	WP_001294626.1	PH domain-containing protein	-
CSC57_RS12565	2468559..2469050	+	492	WP_001205910.1	PH domain-containing protein	-
CSC57_RS12570	2469054..2469413	+	360	WP_000581197.1	holo-ACP synthase	-
CSC57_RS12575	2469479..2470627	+	1149	WP_001281142.1	alanine racemase	-
CSC57_RS12580	2470712..2470882	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
CSC57_RS12585	2470879..2471241	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CSC57_RS12595	2471591..2472592	+	1002	WP_000390831.1	PP2C family protein-serine/threonine phosphatase	-
CSC57_RS12600	2472711..2473037	+	327	WP_001052491.1	anti-sigma factor antagonist	-
CSC57_RS12605	2473039..2473518	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
CSC57_RS12610	2473493..2474263	+	771	WP_001041102.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T106074 WP_000621175.1 NZ_CP029649:2470879-2471241 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T106074 NZ_CP029649:2470879-2471241 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT106074 WP_000948331.1 NZ_CP029649:2470712-2470882 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT106074 NZ_CP029649:2470712-2470882 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1