Detailed information of TA system TA106019

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ImmA-IrrE/HTH_26(antitoxin)
• Location: 2606406..2607101
• Replicon: chromosome
• Accession: NZ_CP029612
• Organism: Enterococcus faecalis strain H25

Toxin (Protein)

• Gene name: IrrE
• Uniprot ID: E2YXR2
• Locus tag: CNQ40_RS13870
• Protein ID: WP_002380839.1
• Coordinates: 2606757..2607101 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: ImmA
• Uniprot ID: -
• Locus tag: CNQ40_RS13865
• Protein ID: WP_002373919.1
• Coordinates: 2606406..2606738 (+)
• Length: 111 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CNQ40_RS13815	2602170..2602985	-	816	WP_002380833.1	PD-(D/E)XK nuclease-like domain-containing protein	-
CNQ40_RS13820	2602948..2603964	-	1017	WP_002380835.1	recombinase RecT	-
CNQ40_RS13830	2604057..2604413	-	357	WP_010774955.1	hypothetical protein	-
CNQ40_RS13835	2604416..2604640	-	225	WP_002367281.1	hypothetical protein	-
CNQ40_RS13840	2604637..2604936	-	300	WP_010706856.1	hypothetical protein	-
CNQ40_RS13845	2605023..2605439	-	417	WP_002380838.1	hypothetical protein	-
CNQ40_RS16650	2605440..2605610	-	171	WP_002405829.1	hypothetical protein	-
CNQ40_RS13855	2605645..2605914	-	270	WP_002367284.1	hypothetical protein	-
CNQ40_RS13860	2605927..2606109	-	183	WP_002358110.1	hypothetical protein	-
CNQ40_RS13865	2606406..2606738	+	333	WP_002373919.1	helix-turn-helix domain-containing protein	Antitoxin
CNQ40_RS13870	2606757..2607101	+	345	WP_002380839.1	ImmA/IrrE family metallo-endopeptidase	Toxin
CNQ40_RS13875	2607159..2607887	+	729	WP_002380840.1	potassium channel family protein	-
CNQ40_RS13880	2608042..2609223	+	1182	WP_002380841.1	site-specific integrase	-
CNQ40_RS13885	2609326..2609475	-	150	WP_002356321.1	50S ribosomal protein L33	-
CNQ40_RS13890	2609601..2611736	-	2136	WP_002359399.1	penicillin-binding protein 2	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2567537..2609223	41686

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• IrrE N-terminal-like domain (21-86)

Antitoxin

• Cro/C1-type helix-turn-helix domain (5-59)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13640.59 Da
• Isoelectric Point: 5.2241

>T106019 WP_002380839.1 NZ_CP029612:2606757-2607101 [Enterococcus faecalis]
MKSIKELVEEYNVELVFTTLNKRACFDPTYGIIFVNQNLTPSEQEEAIYHELKHVKDHVDIMELYKIPAFRSKMESEAEQ
YMFRSLIEKYEGQYNYSNVIAHYNLKMGQEIYLK

Nucleotide

• Length: 345 bp

>T106019 NZ_CP029612:2606757-2607101 [Enterococcus faecalis]
ATGAAAAGTATCAAAGAGTTGGTAGAAGAATATAATGTGGAGTTAGTTTTTACTACTTTGAACAAACGCGCATGTTTCGA
CCCTACCTACGGTATCATATTTGTAAATCAAAATTTAACACCATCAGAACAAGAAGAAGCAATATATCACGAGTTAAAGC
ATGTAAAAGACCATGTGGATATAATGGAATTGTATAAAATTCCTGCTTTTCGTTCTAAGATGGAATCCGAAGCAGAACAA
TATATGTTTAGAAGCTTAATCGAAAAATATGAAGGACAATACAATTATTCAAATGTTATAGCTCATTACAACTTAAAAAT
GGGACAAGAAATTTATTTGAAATAA

Antitoxin

Protein

• Length: 111 a.a.
• Molecular weight: 12734.28 Da
• Isoelectric Point: 5.2423

>AT106019 WP_002373919.1 NZ_CP029612:2606406-2606738 [Enterococcus faecalis]
MNTYEIIKELTKRKKMSIRQLEITLGYSNGYFSKWKKVSPNSEGLQKVADYFNVSVDYLLGRTDNTKATDEKNSDDLDDV
LDNVMSFDGEPLDDHDREVIRAYLKGRFGK

Nucleotide

• Length: 333 bp

>AT106019 NZ_CP029612:2606406-2606738 [Enterococcus faecalis]
ATGAATACTTATGAAATAATAAAAGAGTTGACAAAAAGGAAGAAAATGTCTATTCGACAATTAGAAATTACTCTAGGTTA
CTCAAATGGATATTTTAGCAAGTGGAAAAAAGTTTCTCCAAACTCAGAAGGCCTACAAAAAGTTGCGGACTACTTCAATG
TATCGGTAGATTATCTATTGGGAAGAACTGATAATACTAAAGCAACTGATGAAAAAAATTCTGATGATTTAGATGATGTA
CTGGATAACGTCATGAGTTTTGACGGTGAACCGCTTGATGATCATGACAGAGAAGTTATCCGTGCATATTTAAAGGGTAG
ATTCGGGAAATAA

Structures

Toxin

• AlphaFold DB: A0A0M2A9N7