Detailed information of TA system TA106013

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 79152..79736
• Replicon: plasmid unnamed
• Accession: NZ_CP029611
• Organism: Acinetobacter pittii strain ST220

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: N9JY46
• Locus tag: DLK06_RS21160
• Protein ID: WP_000286963.1
• Coordinates: 79152..79454 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: DLK06_RS21165
• Protein ID: WP_000985610.1
• Coordinates: 79455..79736 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DLK06_RS21130	74826..76034	-	1209	WP_004832869.1	IS256-like element ISAba26 family transposase	-
DLK06_RS21320	76271..76495	+	225	WP_125510319.1	hypothetical protein	-
DLK06_RS21140	76529..76891	+	363	Protein_100	plasmid replication DNA-binding protein	-
DLK06_RS21145	77091..77570	-	480	Protein_101	GIY-YIG nuclease family protein	-
DLK06_RS21150	77624..78556	+	933	WP_002048759.1	IS5 family transposase	-
DLK06_RS21155	78610..78960	-	351	Protein_103	GIY-YIG nuclease family protein	-
DLK06_RS21160	79152..79454	+	303	WP_000286963.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DLK06_RS21165	79455..79736	+	282	WP_000985610.1	putative addiction module antidote protein	Antitoxin
DLK06_RS21170	79940..80812	+	873	WP_006581603.1	restriction endonuclease	-
DLK06_RS21175	81433..81789	+	357	WP_006581604.1	type II toxin-antitoxin system RelE/ParE family toxin	-
DLK06_RS21180	81782..82084	+	303	WP_001140619.1	XRE family transcriptional regulator	-
DLK06_RS21185	82077..82286	+	210	WP_000069471.1	hypothetical protein	-
DLK06_RS21190	82396..82614	-	219	WP_029747731.1	hypothetical protein	-
DLK06_RS21200	83371..83916	-	546	Protein_111	IS5 family transposase	-
DLK06_RS21205	83844..84232	-	389	Protein_112	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul2	-	1..84302	84302
	-	flank	IS/Tn	-	-	83584..83796	212

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-97)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-91)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11545.02 Da
• Isoelectric Point: 4.6838

>T106013 WP_000286963.1 NZ_CP029611:79152-79454 [Acinetobacter pittii]
MYSIYTTEAFDDWFTKLKDQQAKRRIQVRIDRVEDGNFGDTEPVGEGVSELRFFFGPGYRIYYCKQGQRVVILLAGGDKS
TQSKDIKLALQLAQDLEEEL

Nucleotide

• Length: 303 bp

>T106013 NZ_CP029611:79152-79454 [Acinetobacter pittii]
ATGTACTCAATATATACCACAGAAGCCTTTGATGATTGGTTTACCAAGTTAAAAGATCAACAGGCAAAAAGACGCATACA
AGTCCGAATTGATCGGGTTGAAGATGGGAACTTTGGAGATACTGAACCAGTCGGTGAAGGTGTTTCTGAGTTACGTTTCT
TCTTTGGACCTGGCTATAGGATTTATTACTGCAAGCAAGGGCAAAGGGTCGTTATTCTCTTAGCAGGCGGAGATAAGTCT
ACGCAAAGTAAAGATATAAAACTTGCCCTACAATTAGCACAAGATTTAGAGGAGGAGCTGTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10109.67 Da
• Isoelectric Point: 8.0018

>AT106013 WP_000985610.1 NZ_CP029611:79455-79736 [Acinetobacter pittii]
MAIKLRKWDSAEHLKTEEDMQAYLQACIDESNGDAAFIAKALGNIAKAKGMAQLSRDTGLGRESLYKALSGDVNPSFDTV
IKVVKALNLRLAI

Nucleotide

• Length: 282 bp

>AT106013 NZ_CP029611:79455-79736 [Acinetobacter pittii]
ATGGCTATCAAACTTCGTAAATGGGATAGTGCTGAGCATCTTAAAACAGAAGAAGATATGCAAGCTTACCTACAAGCCTG
TATCGATGAATCTAATGGTGATGCTGCATTCATTGCTAAAGCTTTAGGTAACATTGCTAAAGCTAAAGGAATGGCTCAAT
TATCACGAGATACAGGTCTAGGCCGAGAAAGTCTTTACAAAGCACTTTCTGGTGATGTTAACCCTAGTTTTGATACTGTG
ATTAAAGTTGTAAAAGCACTGAATTTACGCTTAGCGATTTAA

Structures

Toxin

• AlphaFold DB: A0A836MGN2