Detailed information of TA system TA106009

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1364961..1365877
• Replicon: chromosome
• Accession: NZ_CP029609
• Organism: Bacillus subtilis subsp. subtilis strain G7

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: DL538_RS07325
• Protein ID: WP_089172519.1
• Coordinates: 1365131..1365877 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: G4EXD8
• Locus tag: DL538_RS07320
• Protein ID: WP_003232646.1
• Coordinates: 1364961..1365131 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DL538_RS07280	1360678..1361007	+	330	WP_085186201.1	XkdW family protein	-
DL538_RS07285	1361004..1361168	+	165	WP_015715740.1	XkdX family protein	-
DL538_RS07290	1361212..1362051	+	840	WP_131227349.1	phage-like element PBSX protein XepA	-
DL538_RS07295	1362104..1362373	+	270	WP_015252265.1	hemolysin XhlA family protein	-
DL538_RS07300	1362386..1362649	+	264	WP_131227350.1	phage holin	-
DL538_RS07305	1362662..1363558	+	897	WP_131227351.1	N-acetylmuramoyl-L-alanine amidase	-
DL538_RS07310	1363635..1364726	+	1092	WP_160243250.1	acyltransferase family protein	-
DL538_RS07315	1364723..1364875	-	153	WP_131228329.1	three component toxin-antitoxin-antitoxin system antitoxin SpoIISC	-
DL538_RS07320	1364961..1365131	-	171	WP_003232646.1	three component toxin-antitoxin-antitoxin system antitoxin SpoIISB	Antitoxin
DL538_RS07325	1365131..1365877	-	747	WP_089172519.1	toxin-antitoxin-antitoxin system toxin SpoIISA	Toxin
DL538_RS07330	1365987..1366988	-	1002	WP_014479569.1	inorganic phosphate transporter	-
DL538_RS07335	1367001..1367618	-	618	WP_003218470.1	DUF47 domain-containing protein	-
DL538_RS07340	1367894..1369210	-	1317	WP_015252262.1	serine/threonine exchanger	-
DL538_RS07345	1369598..1370548	+	951	WP_003232637.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-238)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29121.60 Da
• Isoelectric Point: 4.6191

>T106009 WP_089172519.1 NZ_CP029609:c1365877-1365131 [Bacillus subtilis subsp. subtilis]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMFAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Nucleotide

• Length: 747 bp

>T106009 NZ_CP029609:c1365877-1365131 [Bacillus subtilis subsp. subtilis]
ATGGTTTTATTCTTTCAGATCATGGTCTGGTGCATCGTGGCCGGACTGGGGTTATACGTGTATGCCACGTGGCGTTTCGA
AGCGAAGGTCAAAGAAAAAATGTTCGCCATCCGGAAAACTTGGTATTTGCTGTTTGTTCTGGGCGCTATGGTATACTGGA
CATATGAGCCCACTTCCCTATTTACCCACTGGGAACGGTATCTCATTGTCGCAGTCAGTTTTGCTTTGATTGATGCTTTT
ATCTTCTTAAGTGCATACGTCAAAAAACTGGCCGGCAGCGAGCTTGAAACAGACACAAGAGAAATTCTTGAAGAAAACAA
CGAAATGCTCCACATGTACCTCAATCGGCTGAAAACATACCAATACCTATTGAAAAACGAACCGATCCATGTTTATTATG
GAAGTATAGATGCTTATGCTGAAGGTATTGATAAGCTGCTGAAAACCTATGCTGATAAAATGAACTTAACGGCTTCTCTT
TGCCACTATTCGACACAGGCTGATAAAGACCGGTTAACCGAGCATATGGATGATCCGGCAGATGTACAAACACGGCTCGA
TCGAAAGGATGTTTATTACGACCAATACGGAAAAGTGGTTCTCATCCCTTTTACCATCGAGACACAGAACTATGTCATCA
AGCTGACGTCTGACAGCATTGTCACGGAATTTGATTATTTGCTATTTACGTCATTAACGAGCATATATGATTTGGTGCTG
CCAATTGAGGAGGAAGGTGAAGGATAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6654.72 Da
• Isoelectric Point: 11.0203

>AT106009 WP_003232646.1 NZ_CP029609:c1365131-1364961 [Bacillus subtilis subsp. subtilis]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Nucleotide

• Length: 171 bp

>AT106009 NZ_CP029609:c1365131-1364961 [Bacillus subtilis subsp. subtilis]
ATGGAACGTGCGTTTCAAAACAGATGCGAGCCCAGAGCGGCGAAGCCGTTTAAAATCCTGAAAAAACGTTCAACCACCAG
TGTCGCAAGCTATCAAGTCAGTCCGCATACAGCAAGAATCTTCAAAGAAAACGAACGGCTGATTGACGAGTATAAACGAA
AAAAAGCATGA

Structures

Antitoxin

• AlphaFold DB: G4EXD8