Detailed information of TA system TA106004

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1861308..1861924
• Replicon: chromosome
• Accession: NZ_CP029608
• Organism: Pseudomonas kribbensis strain 46-2

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A345RMN0
• Locus tag: DLD99_RS08685
• Protein ID: WP_065261097.1
• Coordinates: 1861308..1861520 (+)
• Length: 71 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: DLD99_RS08690
• Protein ID: WP_114881918.1
• Coordinates: 1861520..1861924 (+)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DLD99_RS08650	1856898..1857434	+	537	WP_011333088.1	DsbE family thiol:disulfide interchange protein	-
DLD99_RS08655	1857431..1857901	+	471	WP_114881915.1	cytochrome c-type biogenesis protein CcmH	-
DLD99_RS08660	1857898..1859097	+	1200	WP_114881916.1	c-type cytochrome biogenesis protein CcmI	-
DLD99_RS08665	1859123..1859530	+	408	WP_085710507.1	hypothetical protein	-
DLD99_RS08670	1859571..1859642	-	72	Protein_1696	PIN domain-containing protein	-
DLD99_RS08675	1859694..1860161	-	468	WP_108560308.1	helix-turn-helix domain-containing protein	-
DLD99_RS08680	1860246..1861133	-	888	WP_114881917.1	LysR family transcriptional regulator	-
DLD99_RS08685	1861308..1861520	+	213	WP_065261097.1	type II toxin-antitoxin system HicA family toxin	Toxin
DLD99_RS08690	1861520..1861924	+	405	WP_114881918.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
DLD99_RS08695	1861962..1863149	-	1188	WP_114881919.1	MFS transporter	-
DLD99_RS08700	1863393..1864013	+	621	WP_114881920.1	hypothetical protein	-
DLD99_RS08705	1864104..1865102	-	999	WP_085710515.1	sulfate ABC transporter substrate-binding protein	-
DLD99_RS08710	1865207..1866031	-	825	WP_114881921.1	ion transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 71 a.a.
• Molecular weight: 7904.98 Da
• Isoelectric Point: 10.0381

>T106004 WP_065261097.1 NZ_CP029608:1861308-1861520 [Pseudomonas kribbensis]
VQSRLLIKELEEAGWTLDRITGSHHIFTHRYNPYTIPVPHPKKDLPVGTVKSIRRRAGLYNPPASDTGDS

Nucleotide

• Length: 213 bp

>T106004 NZ_CP029608:1861308-1861520 [Pseudomonas kribbensis]
GTGCAGAGCAGGTTATTGATCAAGGAGCTGGAGGAAGCAGGCTGGACGCTGGATCGGATTACCGGCAGTCATCACATCTT
CACGCACCGTTACAACCCTTACACGATTCCCGTTCCTCACCCGAAGAAGGATTTGCCGGTCGGGACGGTCAAGAGCATCA
GGAGGCGCGCCGGGTTATACAACCCGCCAGCCAGTGACACAGGAGATTCGTAA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14846.83 Da
• Isoelectric Point: 4.3841

>AT106004 WP_114881918.1 NZ_CP029608:1861520-1861924 [Pseudomonas kribbensis]
MQYPICIEWGDENTAIGIQIPDIPGAVTAGDTFEDAYNAAVEIAHIMLQEIAADGDPIPMPTSAATHRNNPEFADMGWGM
LELDISPYMGKTEKVNVTLPGYVIQRIDRYVREHNVKSRSSFLADAAMEKLVRH

Nucleotide

• Length: 405 bp

>AT106004 NZ_CP029608:1861520-1861924 [Pseudomonas kribbensis]
ATGCAATACCCAATCTGCATCGAGTGGGGCGACGAGAACACCGCCATCGGTATTCAGATCCCCGATATTCCCGGCGCGGT
AACGGCCGGTGATACGTTTGAGGATGCCTACAACGCAGCCGTAGAGATTGCCCACATCATGCTGCAGGAGATCGCGGCGG
ATGGTGACCCGATTCCGATGCCGACCTCGGCGGCCACTCATCGCAACAACCCGGAATTTGCCGACATGGGCTGGGGCATG
CTGGAGCTGGATATTTCGCCGTACATGGGCAAGACCGAAAAGGTCAACGTGACGTTGCCCGGTTATGTCATCCAGCGTAT
CGACCGCTATGTGCGCGAGCACAACGTCAAAAGTCGTTCATCCTTTCTGGCGGATGCCGCCATGGAAAAACTGGTCCGGC
ATTGA

Structures

Toxin

• AlphaFold DB: A0A345RMN0