Detailed information of TA system TA106003

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 284216..284859
• Replicon: chromosome
• Accession: NZ_CP029608
• Organism: Pseudomonas kribbensis strain 46-2

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A345RIP1
• Locus tag: DLD99_RS01260
• Protein ID: WP_065261145.1
• Coordinates: 284216..284398 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: DLD99_RS01265
• Protein ID: WP_170931616.1
• Coordinates: 284434..284859 (+)
• Length: 142 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DLD99_RS01240	279291..280295	-	1005	WP_114881005.1	D-cysteine desulfhydrase	-
DLD99_RS01245	280486..281412	+	927	WP_114881006.1	serine acetyltransferase	-
DLD99_RS01250	281701..283662	+	1962	WP_167443790.1	choline transporter BetT	-
DLD99_RS01255	283687..283947	-	261	WP_114881008.1	hypothetical protein	-
DLD99_RS01260	284216..284398	+	183	WP_065261145.1	type II toxin-antitoxin system HicA family toxin	Toxin
DLD99_RS01265	284434..284859	+	426	WP_170931616.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
DLD99_RS01270	284882..285919	-	1038	WP_114881009.1	LacI family DNA-binding transcriptional regulator	-
DLD99_RS01275	286126..288351	+	2226	WP_114881010.1	TonB-dependent receptor	-
DLD99_RS01280	288353..289672	+	1320	WP_114881011.1	LLM class flavin-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (11-135)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6836.96 Da
• Isoelectric Point: 10.5417

>T106003 WP_065261145.1 NZ_CP029608:284216-284398 [Pseudomonas kribbensis]
MRSREMIRMIEEDGWYLVAVKGSHHQYKHPGKPGRVTIKHPDSDLPKGTINSILKQAGLK

Nucleotide

• Length: 183 bp

>T106003 NZ_CP029608:284216-284398 [Pseudomonas kribbensis]
ATGCGTAGTCGGGAAATGATCAGGATGATCGAGGAGGATGGATGGTATCTGGTCGCCGTGAAAGGCAGTCACCACCAATA
CAAACATCCGGGCAAACCAGGGAGGGTGACGATCAAGCATCCGGATTCGGATCTTCCCAAGGGAACGATCAACAGCATCT
TGAAACAGGCGGGTTTGAAATGA

Antitoxin

Protein

• Length: 142 a.a.
• Molecular weight: 15486.28 Da
• Isoelectric Point: 4.2513

>AT106003 WP_170931616.1 NZ_CP029608:284434-284859 [Pseudomonas kribbensis]
MSEEGFTYMKFPVVLHKDADSDYGVIVPDVPGCFSAGTTVAEAFDNTQEALALHYEGLVADGAPLPRVQDIDAHLENPDY
AGGIWGVVEFDITPYFGKSVRFNATLPEQLLERIDQTVRRDQRYSSRSGFLAAAALRELSA

Nucleotide

• Length: 426 bp

>AT106003 NZ_CP029608:284434-284859 [Pseudomonas kribbensis]
ATGTCAGAGGAAGGATTCACTTACATGAAATTCCCGGTCGTTCTGCACAAGGATGCCGACTCAGACTACGGAGTGATCGT
CCCGGATGTACCGGGCTGCTTCTCCGCAGGCACTACGGTAGCCGAAGCTTTTGACAATACTCAGGAGGCACTGGCCTTGC
ACTATGAGGGACTGGTCGCTGATGGCGCACCGTTGCCTCGGGTTCAGGACATTGATGCCCATCTCGAGAATCCGGATTAC
GCCGGCGGTATCTGGGGTGTGGTCGAGTTTGATATCACTCCCTATTTCGGCAAGTCGGTGCGCTTCAACGCGACTCTGCC
GGAGCAGTTGCTGGAGCGTATTGACCAGACAGTACGACGTGATCAGCGTTACAGCTCTCGCTCCGGATTTTTGGCTGCTG
CTGCCTTGCGTGAATTGTCAGCTTAA

Structures

Toxin

• AlphaFold DB: A0A345RIP1