Detailed information of TA system TA106000

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 5781964..5782550
• Replicon: chromosome
• Accession: NZ_CP029605
• Organism: Pseudomonas aeruginosa strain 24Pae112

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: CTT40_RS28025
• Protein ID: WP_003120987.1
• Coordinates: 5782251..5782550 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: CTT40_RS28020
• Protein ID: WP_003448662.1
• Coordinates: 5781964..5782254 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CTT40_RS28005	5777546..5779435	+	1890	WP_003448700.1	hypothetical protein	-
CTT40_RS28010	5779432..5781408	+	1977	WP_096316534.1	DEAD/DEAH box helicase	-
CTT40_RS28015	5781549..5781893	+	345	WP_016851612.1	hypothetical protein	-
CTT40_RS28020	5781964..5782254	-	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
CTT40_RS28025	5782251..5782550	-	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CTT40_RS28030	5782752..5783876	+	1125	WP_012076859.1	TcpQ domain-containing protein	-
CTT40_RS28035	5783876..5785585	+	1710	WP_003099757.1	PilN family type IVB pilus formation outer membrane protein	-
CTT40_RS28040	5785589..5786914	+	1326	WP_003099758.1	type 4b pilus protein PilO2	-
CTT40_RS28045	5786904..5787437	+	534	WP_003099760.1	type IV pilus biogenesis protein PilP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	5752794..5854462	101668

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T106000 WP_003120987.1 NZ_CP029605:c5782550-5782251 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T106000 NZ_CP029605:c5782550-5782251 [Pseudomonas aeruginosa]
ATGGTCGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT106000 WP_003448662.1 NZ_CP029605:c5782254-5781964 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT106000 NZ_CP029605:c5782254-5781964 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGACAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
TTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCCCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCTACATTGAAAACCATGCTGGCA
GTAATGCGAGCTCTGGGAGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA

Structures

Toxin

• AlphaFold DB: G8CP73