Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | mbcAT/COG5642-COG5654 |
| Location | 2232739..2233637 | Replicon | chromosome |
| Accession | NC_000962 | ||
| Organism | Mycobacterium tuberculosis H37Rv | ||
Toxin (Protein)
| Gene name | mbcT | Uniprot ID | P9WLP9 |
| Locus tag | Rv1989c | Protein ID | 15609126 |
| Coordinates | 2232739..2233299 (-) | Length | 187 a.a. |
Antitoxin (Protein)
| Gene name | mbcA | Uniprot ID | P9WLP7 |
| Locus tag | Rv1990c | Protein ID | 15609127 |
| Coordinates | 2233296..2233637 (-) | Length | 114 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| Rv1984c | 2227908..2228561 | - | 654 | NP_216500.1 | cutinase | - |
| RV1984a | 2228676..2228906 | + | 231 | YP_009030034.1 | hypothetical protein | - |
| Rv1985c | 2228991..2229902 | - | 912 | NP_216501.1 | HTH-type transcriptional regulator | - |
| Rv1986 | 2230011..2230610 | + | 600 | NP_216502.1 | amino acid transporter | - |
| Rv1987 | 2231026..2231454 | + | 429 | NP_216503.1 | chitinase | - |
| Rv1988 | 2231680..2232219 | + | 540 | NP_216504.1 | 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37) | - |
| Rv1989c | 2232739..2233299 | - | 561 | NP_216505.1 | hypothetical protein | Toxin |
| Rv1990c | 2233296..2233637 | - | 342 | NP_216506.1 | transcriptional regulator | Antitoxin |
| Rv1991c | 2234305..2234649 | - | 345 | NP_216507.1 | mRNA interferase MazF6 | - |
| Rv1991A | 2234643..2234891 | - | 249 | YP_007410673.1 | antitoxin MazE6 | - |
| Rv1992c | 2234991..2237306 | - | 2316 | NP_216508.1 | cation transporter ATPase G | - |
| Rv1993c | 2237303..2237575 | - | 273 | NP_216509.1 | hypothetical protein | - |
| Rv1994c | 2237628..2237984 | - | 357 | NP_216510.1 | HTH-type transcriptional regulator CmtR | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 187 a.a. Molecular weight: 20248.70 Da Isoelectric Point: 4.5091
>T106 15609126 NC_000962:c2233299-2232739 [Mycobacterium tuberculosis H37Rv]
VSDALDEGLVQRIDARGTIEWSETCYRYTGAHRDALSGEGARRFGGRWNPPLLFPAIYLADSAQACMVEVERAAQAASTT
AEKMLEAAYRLHTIDVTDLAVLDLTTPQAREAVGLENDDIYGDDWSGCQAVGHAAWFLHMQGVLVPAAGGVGLVVTAYEQ
RTRPGQLQLRQSVDLTPALYQELRAT
VSDALDEGLVQRIDARGTIEWSETCYRYTGAHRDALSGEGARRFGGRWNPPLLFPAIYLADSAQACMVEVERAAQAASTT
AEKMLEAAYRLHTIDVTDLAVLDLTTPQAREAVGLENDDIYGDDWSGCQAVGHAAWFLHMQGVLVPAAGGVGLVVTAYEQ
RTRPGQLQLRQSVDLTPALYQELRAT
Download Length: 561 bp
>T106 NC_000962:c2233299-2232739 [Mycobacterium tuberculosis H37Rv]
GTGAGCGATGCCCTCGATGAAGGGCTCGTCCAGCGTATCGACGCACGCGGAACAATTGAGTGGTCGGAAACGTGCTACCG
GTATACCGGCGCGCACCGTGACGCCTTGTCCGGTGAGGGCGCGCGCAGATTCGGAGGCAGGTGGAATCCGCCGCTGCTCT
TTCCGGCGATCTATCTTGCTGATTCCGCCCAAGCCTGCATGGTTGAGGTGGAACGGGCGGCGCAAGCGGCTTCAACGACC
GCAGAGAAGATGCTCGAGGCGGCCTACCGACTACACACGATCGACGTCACGGACCTGGCCGTCCTCGATCTGACAACCCC
GCAAGCTCGGGAAGCCGTGGGGCTCGAGAACGACGACATCTATGGCGACGACTGGTCAGGGTGCCAGGCGGTCGGACATG
CGGCCTGGTTCTTGCACATGCAAGGTGTCCTCGTGCCGGCGGCGGGCGGTGTCGGCCTCGTTGTCACCGCGTATGAACAG
CGAACTCGGCCGGGCCAACTACAACTGCGACAAAGCGTCGATCTGACGCCTGCTCTTTACCAAGAACTTCGAGCCACGTA
G
GTGAGCGATGCCCTCGATGAAGGGCTCGTCCAGCGTATCGACGCACGCGGAACAATTGAGTGGTCGGAAACGTGCTACCG
GTATACCGGCGCGCACCGTGACGCCTTGTCCGGTGAGGGCGCGCGCAGATTCGGAGGCAGGTGGAATCCGCCGCTGCTCT
TTCCGGCGATCTATCTTGCTGATTCCGCCCAAGCCTGCATGGTTGAGGTGGAACGGGCGGCGCAAGCGGCTTCAACGACC
GCAGAGAAGATGCTCGAGGCGGCCTACCGACTACACACGATCGACGTCACGGACCTGGCCGTCCTCGATCTGACAACCCC
GCAAGCTCGGGAAGCCGTGGGGCTCGAGAACGACGACATCTATGGCGACGACTGGTCAGGGTGCCAGGCGGTCGGACATG
CGGCCTGGTTCTTGCACATGCAAGGTGTCCTCGTGCCGGCGGCGGGCGGTGTCGGCCTCGTTGTCACCGCGTATGAACAG
CGAACTCGGCCGGGCCAACTACAACTGCGACAAAGCGTCGATCTGACGCCTGCTCTTTACCAAGAACTTCGAGCCACGTA
G
Antitoxin
Download Length: 114 a.a. Molecular weight: 12488.33 Da Isoelectric Point: 4.8359
>AT106 15609127 NC_000962:c2233637-2233296 [Mycobacterium tuberculosis H37Rv]
MGVNVLASTVSGAIERLGLTYEEVGDIVDASPRSVARWTAGQVVPQRLNKQRLIELAYVADALAEVLPRDQANVWMFSPN
RLLEHRKPADLVRDGEYQRVLALIDAMAEGVFV
MGVNVLASTVSGAIERLGLTYEEVGDIVDASPRSVARWTAGQVVPQRLNKQRLIELAYVADALAEVLPRDQANVWMFSPN
RLLEHRKPADLVRDGEYQRVLALIDAMAEGVFV
Download Length: 342 bp
>AT106 NC_000962:c2233637-2233296 [Mycobacterium tuberculosis H37Rv]
ATGGGAGTAAATGTGCTCGCCTCGACCGTGTCGGGTGCGATCGAGCGCTTGGGATTGACCTACGAGGAAGTCGGTGACAT
CGTCGATGCCTCGCCGCGTTCCGTGGCGCGATGGACCGCAGGTCAGGTGGTTCCCCAACGCCTCAACAAGCAACGACTTA
TCGAGCTGGCCTATGTCGCCGACGCCCTCGCGGAAGTGCTGCCGCGTGACCAGGCGAACGTGTGGATGTTTTCGCCGAAT
CGGTTACTGGAACACCGCAAGCCTGCCGACCTCGTGCGAGACGGCGAGTACCAACGCGTGTTGGCGCTCATCGACGCGAT
GGCGGAGGGAGTGTTCGTGTGA
ATGGGAGTAAATGTGCTCGCCTCGACCGTGTCGGGTGCGATCGAGCGCTTGGGATTGACCTACGAGGAAGTCGGTGACAT
CGTCGATGCCTCGCCGCGTTCCGTGGCGCGATGGACCGCAGGTCAGGTGGTTCCCCAACGCCTCAACAAGCAACGACTTA
TCGAGCTGGCCTATGTCGCCGACGCCCTCGCGGAAGTGCTGCCGCGTGACCAGGCGAACGTGTGGATGTTTTCGCCGAAT
CGGTTACTGGAACACCGCAAGCCTGCCGACCTCGTGCGAGACGGCGAGTACCAACGCGTGTTGGCGCTCATCGACGCGAT
GGCGGAGGGAGTGTTCGTGTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
References
(1) Diana Mendes Freire et al. (2019) An NAD+ Phosphorylase Toxin Triggers Mycobacterium tuberculosis Cell Death. Molecular Cell 73(6):1282-1291.e8. [PubMed:30792174]
(2) Ragnhild Bager Skjerning et al. (2019) The RES domain toxins of RES-Xre toxin-antitoxin modules induce cell stasis by degrading NAD+. Molecular Microbiology 111(1):221-236. [PubMed:30315706]
(3) Kanchiyaphat Ariyachaokun et al. (2020) Multi-Stress Induction of the Mycobacterium tuberculosis MbcTA Bactericidal Toxin-Antitoxin System. Toxins 12(5):329. [PubMed:32429486]