Detailed information of TA system TA105997

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 2788386..2789428
• Replicon: chromosome
• Accession: NZ_CP029605
• Organism: Pseudomonas aeruginosa strain 24Pae112

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: CTT40_RS13685
• Protein ID: WP_003109777.1
• Coordinates: 2788853..2789428 (+)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: I3TV68
• Locus tag: CTT40_RS13680
• Protein ID: WP_003050245.1
• Coordinates: 2788386..2788856 (+)
• Length: 157 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CTT40_RS13645	2783778..2785196	-	1419	WP_020924934.1	TIGR03752 family integrating conjugative element protein	-
CTT40_RS13650	2785186..2786097	-	912	WP_003105643.1	TIGR03749 family integrating conjugative element protein	-
CTT40_RS13655	2786094..2786786	-	693	WP_003105641.1	TIGR03746 family integrating conjugative element protein	-
CTT40_RS13660	2786783..2787181	-	399	WP_003105639.1	TIGR03750 family conjugal transfer protein	-
CTT40_RS13665	2787193..2787552	-	360	WP_003090173.1	TIGR03745 family integrating conjugative element membrane protein	-
CTT40_RS13670	2787569..2787802	-	234	WP_003050225.1	TIGR03758 family integrating conjugative element protein	-
CTT40_RS13675	2787799..2788182	-	384	WP_003120001.1	RAQPRD family integrative conjugative element protein	-
CTT40_RS13680	2788386..2788856	+	471	WP_003050245.1	helix-turn-helix domain-containing protein	Antitoxin
CTT40_RS13685	2788853..2789428	+	576	WP_003109777.1	PIN domain-containing protein	Toxin
CTT40_RS13690	2789446..2790360	+	915	WP_003105629.1	AAA family ATPase	-
CTT40_RS13695	2790357..2790827	+	471	WP_003105626.1	hypothetical protein	-
CTT40_RS13700	2790824..2791324	+	501	WP_003090159.1	hypothetical protein	-
CTT40_RS13705	2791324..2792226	+	903	WP_003105624.1	nucleotidyltransferase domain-containing protein	-
CTT40_RS13710	2792265..2792990	+	726	WP_003050273.1	CBASS effector endonuclease NucC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	blaKPC-2	-	2724237..2841241	117004

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-114)

Antitoxin

• Helix-turn-helix domain, group 17 (85-133)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21644.79 Da
• Isoelectric Point: 5.6172

>T105997 WP_003109777.1 NZ_CP029605:2788853-2789428 [Pseudomonas aeruginosa]
MRHSPFTAVYDACVLYPAPLRDFLMWLGLSGRFRARWSQAIHEEWKRNLLINRPDLTRVQVDRTSDLMDRAIPDGLVEGY
EALVAGLTLPDPDDRHVLAAAIRCGASVIVTFNERDFPNDLLAPYGIESQHPDEFVDNLLDLDAAAVVSAAQRQRAQLKH
PPIEVDRYLEILLRQGLVQTTKVLATYRTIL

Nucleotide

• Length: 576 bp

>T105997 NZ_CP029605:2788853-2789428 [Pseudomonas aeruginosa]
ATGAGGCATTCCCCATTCACCGCCGTCTACGACGCGTGCGTTCTATATCCCGCGCCACTGCGGGATTTCCTGATGTGGCT
CGGCCTGTCTGGCCGCTTCCGGGCGCGGTGGAGCCAAGCCATTCATGAGGAGTGGAAACGCAACCTGTTGATCAACCGCC
CCGATCTCACCCGGGTTCAGGTCGACAGGACGTCGGATCTCATGGACCGGGCAATTCCGGACGGCTTGGTGGAGGGCTAC
GAAGCGCTCGTGGCAGGCCTGACATTGCCCGATCCGGACGACCGGCACGTCCTGGCTGCGGCGATTCGCTGCGGTGCGAG
CGTGATTGTGACGTTCAACGAACGGGATTTCCCGAACGATCTGCTGGCTCCGTACGGCATCGAATCGCAGCACCCCGATG
AGTTTGTGGACAACCTACTGGATCTGGACGCGGCCGCCGTGGTGTCGGCTGCGCAGCGCCAGCGTGCCCAACTCAAGCAT
CCGCCGATCGAGGTGGACCGCTATCTCGAAATCCTGCTGCGCCAGGGCCTTGTGCAAACGACCAAGGTGCTGGCGACCTA
TCGCACCATTCTCTGA

Antitoxin

Protein

• Length: 157 a.a.
• Molecular weight: 17245.62 Da
• Isoelectric Point: 6.3803

>AT105997 WP_003050245.1 NZ_CP029605:2788386-2788856 [Pseudomonas aeruginosa]
MTTATAQSKMTLPAAGEVKAAVQGQRALAAYLATQFETQHIQIFDDHKQAHQVELPTSALRLLVDILAELADGNAVKVVP
VHAELTTQEAADLLNVSRPHFVKLLEDGVLAFHRTGKHRRVRFADLMQYKEARERASEQAMAELAQQSQELGMGYE

Nucleotide

• Length: 471 bp

>AT105997 NZ_CP029605:2788386-2788856 [Pseudomonas aeruginosa]
ATGACCACCGCCACTGCCCAATCCAAGATGACCCTTCCCGCCGCGGGAGAGGTGAAGGCAGCCGTCCAGGGTCAACGTGC
CTTGGCGGCTTACCTCGCAACCCAGTTCGAGACGCAGCACATCCAGATCTTTGATGACCACAAACAGGCTCATCAGGTGG
AACTGCCTACCTCGGCTTTACGCCTGCTGGTCGACATCCTGGCCGAGTTGGCCGATGGCAATGCAGTAAAGGTGGTGCCC
GTCCATGCAGAGCTGACGACCCAAGAGGCGGCGGACTTGCTCAACGTGTCCCGTCCCCATTTCGTCAAGCTGCTCGAAGA
TGGGGTGTTGGCATTTCATCGCACCGGCAAGCACCGCAGGGTGAGGTTCGCCGATCTGATGCAGTACAAGGAAGCGCGCG
AGCGCGCCAGTGAGCAGGCGATGGCCGAACTCGCTCAGCAGTCGCAAGAGTTGGGAATGGGATACGAATGA

Structures

Antitoxin

• AlphaFold DB: I3TV68