Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 102692..103428 Replicon plasmid pDA33145-152
Accession NZ_CP029598
Organism Klebsiella quasipneumoniae subsp. similipneumoniae strain ATCC 700603

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag DLJ73_RS27035 Protein ID WP_003026803.1
Coordinates 102946..103428 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag DLJ73_RS27030 Protein ID WP_003026799.1
Coordinates 102692..102958 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DLJ73_RS26990 98536..98898 - 363 WP_004206665.1 arsenite efflux transporter metallochaperone ArsD -
DLJ73_RS26995 98950..99300 - 351 WP_004206664.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
DLJ73_RS27000 99658..99906 + 249 WP_004193994.1 hypothetical protein -
DLJ73_RS27005 99903..101114 + 1212 WP_032415728.1 hypothetical protein -
DLJ73_RS27010 101248..101739 + 492 WP_004206662.1 hypothetical protein -
DLJ73_RS27015 101800..102003 + 204 WP_004206661.1 hemolysin expression modulator Hha -
DLJ73_RS27020 102017..102247 + 231 WP_004206660.1 hypothetical protein -
DLJ73_RS27030 102692..102958 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
DLJ73_RS27035 102946..103428 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
DLJ73_RS27045 103723..104691 - 969 WP_072500845.1 IS5-like element IS903B family transposase -
DLJ73_RS27050 104747..104854 + 108 WP_086071112.1 transposase domain-containing protein -
DLJ73_RS27055 104888..105328 - 441 WP_004206983.1 VGR-related protein -
DLJ73_RS27060 105630..106442 - 813 WP_003032093.1 PfkB domain-containing protein -
DLJ73_RS27065 106455..107429 - 975 WP_003032095.1 SIS domain-containing protein -
DLJ73_RS27070 107434..108219 - 786 WP_003032097.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - mrkA / mrkB / mrkC / mrkF / mrkJ 1..151998 151998
- flank IS/Tn - - 103723..104691 968


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T105988 WP_003026803.1 NZ_CP029598:102946-103428 [Klebsiella quasipneumoniae subsp. similipneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T105988 NZ_CP029598:102946-103428 [Klebsiella quasipneumoniae subsp. similipneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGCAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT105988 WP_003026799.1 NZ_CP029598:102692-102958 [Klebsiella quasipneumoniae subsp. similipneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT105988 NZ_CP029598:102692-102958 [Klebsiella quasipneumoniae subsp. similipneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References