Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 60831..61474 Replicon plasmid pDA33145-152
Accession NZ_CP029598
Organism Klebsiella quasipneumoniae subsp. similipneumoniae strain ATCC 700603

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag DLJ73_RS26805 Protein ID WP_001044770.1
Coordinates 61058..61474 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag DLJ73_RS26800 Protein ID WP_001261282.1
Coordinates 60831..61061 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DLJ73_RS26780 56896..58269 + 1374 WP_004206605.1 hypothetical protein -
DLJ73_RS26785 58454..59782 + 1329 WP_004206607.1 nucleotidyltransferase -
DLJ73_RS26790 59790..60365 + 576 WP_004206608.1 SLATT domain-containing protein -
DLJ73_RS26795 60632..60874 - 243 Protein_62 hypothetical protein -
DLJ73_RS26800 60831..61061 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
DLJ73_RS26805 61058..61474 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
DLJ73_RS26810 61548..63110 + 1563 WP_004206609.1 AAA family ATPase -
DLJ73_RS26815 63095..64117 + 1023 WP_000361404.1 helicase UvrD -
DLJ73_RS26820 64661..65569 + 909 WP_032426221.1 HNH endonuclease -
DLJ73_RS26825 65755..66105 - 351 WP_004187110.1 DUF305 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - mrkA / mrkB / mrkC / mrkF / mrkJ 1..151998 151998


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T105987 WP_001044770.1 NZ_CP029598:61058-61474 [Klebsiella quasipneumoniae subsp. similipneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T105987 NZ_CP029598:61058-61474 [Klebsiella quasipneumoniae subsp. similipneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT105987 WP_001261282.1 NZ_CP029598:60831-61061 [Klebsiella quasipneumoniae subsp. similipneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT105987 NZ_CP029598:60831-61061 [Klebsiella quasipneumoniae subsp. similipneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References