Detailed information of TA system TA105970

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 203106..203851
• Replicon: plasmid pDA33144-220
• Accession: NZ_CP029591
• Organism: Klebsiella pneumoniae strain DA33144

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A331KSM6
• Locus tag: DLJ67_RS27770
• Protein ID: WP_032408901.1
• Coordinates: 203106..203597 (-)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: DLJ67_RS27775
• Protein ID: WP_014386183.1
• Coordinates: 203585..203851 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DLJ67_RS27745	198790..199545	+	756	WP_032408898.1	DeoR/GlpR family DNA-binding transcription regulator	-
DLJ67_RS27750	199807..200247	+	441	WP_014386179.1	IS66 family insertion sequence hypothetical protein	-
DLJ67_RS27755	200244..200594	+	351	WP_014386180.1	IS66 family insertion sequence element accessory protein TnpB	-
DLJ67_RS27760	200625..202217	+	1593	Protein_209	IS66 family transposase	-
DLJ67_RS27765	202414..202665	+	252	WP_032408900.1	aldo/keto reductase	-
DLJ67_RS27770	203106..203597	-	492	WP_032408901.1	GNAT family N-acetyltransferase	Toxin
DLJ67_RS27775	203585..203851	-	267	WP_014386183.1	DUF1778 domain-containing protein	Antitoxin
DLJ67_RS27780	204048..204206	+	159	WP_014386184.1	type I toxin-antitoxin system Hok family toxin	-
DLJ67_RS27785	204450..204935	-	486	WP_014386185.1	hypothetical protein	-
DLJ67_RS27790	205125..205397	+	273	WP_032408902.1	hypothetical protein	-
DLJ67_RS27795	205394..205744	+	351	WP_014386186.1	hypothetical protein	-
DLJ67_RS27810	206381..206707	+	327	WP_014386187.1	hypothetical protein	-
DLJ67_RS27815	206769..206981	+	213	WP_014386188.1	hypothetical protein	-
DLJ67_RS27820	206992..207216	+	225	WP_014386189.1	hypothetical protein	-
DLJ67_RS27825	207297..207617	+	321	WP_014386190.1	type II toxin-antitoxin system RelE/ParE family toxin	-
DLJ67_RS27830	207607..207885	+	279	WP_004152721.1	helix-turn-helix domain-containing protein	-
DLJ67_RS27835	207886..208299	+	414	WP_013023817.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	mph(A) / sul1 / qacE / aadA2 / dfrA12 / blaTEM-1B / blaCTX-M-15 / aac(6')-Ib-cr / blaOXA-1 / tet(A)	-	1..219996	219996

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (89-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-87)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17667.46 Da
• Isoelectric Point: 8.8757

>T105970 WP_032408901.1 NZ_CP029591:c203597-203106 [Klebsiella pneumoniae]
VGKVTAPVPLGTSHILAEFHCGEPVLDEWIKHRGLKNQSLGAARTFVVCRGNASQVLAYYSLATGSVTHAIAPGSLRRNM
PDPVPVIILARLAVDTSYHGQGLGADLLHDAVLRCCRVAENIGVRAVMVHALSDSARQFYIHHGFIPSQTQDRTLFLRLP
LIT

Nucleotide

• Length: 492 bp

>T105970 NZ_CP029591:c203597-203106 [Klebsiella pneumoniae]
GTGGGCAAAGTAACGGCTCCGGTCCCACTCGGTACGTCACACATCCTCGCTGAGTTTCACTGTGGTGAGCCTGTACTGGA
CGAATGGATAAAGCACCGGGGACTAAAAAATCAGTCCCTGGGGGCGGCCAGAACGTTTGTGGTGTGCCGGGGAAATGCTT
CACAGGTGCTTGCCTACTACTCTCTCGCGACCGGCAGCGTCACTCATGCCATTGCGCCGGGAAGCCTGCGAAGGAATATG
CCCGATCCTGTACCTGTCATCATCCTGGCCCGTCTTGCTGTCGATACGAGTTATCACGGCCAGGGACTGGGGGCTGATTT
ACTGCATGATGCCGTATTACGTTGCTGTCGGGTGGCTGAGAACATTGGCGTGCGGGCCGTTATGGTTCATGCACTCTCTG
ACAGTGCCAGACAGTTCTACATACATCACGGATTTATCCCTTCACAAACGCAGGATAGAACCCTTTTTTTACGGTTGCCT
CTCATTACTTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10090.66 Da
• Isoelectric Point: 5.1169

>AT105970 WP_014386183.1 NZ_CP029591:c203851-203585 [Klebsiella pneumoniae]
MKSDVQLNIRAKEAQRALIDTAAEILHKSRTDFILEMACQAAENVILDRRMFNLNDEQYAEFIDMLDAPVTANPAMEKLL
ARKPQWAK

Nucleotide

• Length: 267 bp

>AT105970 NZ_CP029591:c203851-203585 [Klebsiella pneumoniae]
ATGAAATCTGATGTTCAGCTCAACATCCGGGCGAAAGAAGCCCAGCGGGCTCTGATTGATACTGCCGCTGAGATTCTCCA
CAAATCACGCACTGATTTTATACTGGAGATGGCCTGTCAGGCGGCGGAAAACGTCATTCTTGACCGCCGTATGTTCAATC
TCAATGATGAACAGTACGCCGAGTTTATCGACATGCTGGATGCGCCGGTCACCGCTAACCCGGCGATGGAGAAACTCCTG
GCGAGAAAACCGCAGTGGGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A331KSM6