Detailed information of TA system TA105969

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 179312..179955
• Replicon: plasmid pDA33144-220
• Accession: NZ_CP029591
• Organism: Klebsiella pneumoniae strain DA33144

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: DLJ67_RS27625
• Protein ID: WP_014386165.1
• Coordinates: 179312..179728 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: DLJ67_RS27630
• Protein ID: WP_032408893.1
• Coordinates: 179725..179955 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DLJ67_RS27600	175932..176363	+	432	WP_014386161.1	conjugation system SOS inhibitor PsiB	-
DLJ67_RS27605	176360..177088	+	729	WP_014386162.1	plasmid SOS inhibition protein A	-
DLJ67_RS27610	177085..177411	+	327	WP_004152639.1	hypothetical protein	-
DLJ67_RS27615	177467..177841	+	375	WP_032408891.1	hypothetical protein	-
DLJ67_RS27620	178021..178989	-	969	WP_074422984.1	IS5 family transposase	-
DLJ67_RS27625	179312..179728	-	417	WP_014386165.1	PIN domain-containing protein	Toxin
DLJ67_RS27630	179725..179955	-	231	WP_032408893.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
DLJ67_RS27640	180275..180709	+	435	WP_000103648.1	RidA family protein	-
DLJ67_RS27645	180714..181388	+	675	WP_014386166.1	DUF1028 domain-containing protein	-
DLJ67_RS27650	181404..182561	+	1158	WP_032408895.1	acetylornithine deacetylase	-
DLJ67_RS27655	182732..183880	+	1149	WP_014386168.1	ABC transporter ATP-binding protein	-
DLJ67_RS27660	183887..184882	+	996	WP_000611945.1	NAD(P)H-quinone oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	mph(A) / sul1 / qacE / aadA2 / dfrA12 / blaTEM-1B / blaCTX-M-15 / aac(6')-Ib-cr / blaOXA-1 / tet(A)	-	1..219996	219996
	-	flank	IS/Tn	-	-	178021..178989	968

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-130)

Antitoxin

• SpoVT-AbrB domain (7-55)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15127.44 Da
• Isoelectric Point: 7.8841

>T105969 WP_014386165.1 NZ_CP029591:c179728-179312 [Klebsiella pneumoniae]
MTKTYMLDTNICSYIMREQPEAVLKRLEQAVLRRHRIVVSAITYAEMRFGAIGKRASPRHVQLVDAFCARLDAILAWDRA
AVDATTEVKATLAAAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVAGLTFEDWVQ

Nucleotide

• Length: 417 bp

>T105969 NZ_CP029591:c179728-179312 [Klebsiella pneumoniae]
ATGACCAAAACCTACATGCTGGATACTAATATCTGCTCTTATATCATGCGCGAACAACCGGAAGCGGTGCTGAAACGCCT
AGAGCAAGCGGTGTTGCGCCGTCACCGGATAGTGGTGTCAGCCATCACCTATGCCGAAATGCGTTTTGGTGCCATCGGTA
AAAGGGCTTCGCCCCGTCATGTGCAGCTGGTGGACGCATTCTGTGCGCGCCTTGATGCCATACTGGCTTGGGATCGGGCC
GCAGTTGACGCTACAACAGAGGTTAAGGCCACGCTAGCAGCTGCCGGCACGCCAATCGGCCCAAACGACACAGCCATTGC
TGGACATGCCATTGCCGCTGGCGCCATCCTGGTGACGAATAATACGCGTGAATTTGAGCGAGTCGCTGGTCTGACGTTTG
AAGACTGGGTGCAATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8854.89 Da
• Isoelectric Point: 4.5112

>AT105969 WP_032408893.1 NZ_CP029591:c179955-179725 [Klebsiella pneumoniae]
MRTVSVFRNGNNRAIRLPRDLDFDGVNELEIIREGDTLILRPVKPSWSSFRQEEKADADFMAERENVVSDEGRFEL

Nucleotide

• Length: 231 bp

>AT105969 NZ_CP029591:c179955-179725 [Klebsiella pneumoniae]
ATGCGAACTGTATCTGTATTCAGGAACGGTAATAACCGGGCCATCCGTCTGCCCCGCGATCTGGACTTTGACGGCGTGAA
CGAACTGGAAATCATCCGCGAGGGCGATACCCTCATCCTGCGGCCGGTAAAACCCAGTTGGAGTTCGTTCCGCCAGGAGG
AAAAAGCTGACGCAGATTTTATGGCGGAGCGTGAAAATGTTGTTAGTGACGAGGGACGTTTTGAGCTATGA