Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 104871..105607 Replicon plasmid pDA33144-220
Accession NZ_CP029591
Organism Klebsiella pneumoniae strain DA33144

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag DLJ67_RS27185 Protein ID WP_003026803.1
Coordinates 104871..105353 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag DLJ67_RS27190 Protein ID WP_003026799.1
Coordinates 105341..105607 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DLJ67_RS27170 100508..101470 + 963 WP_004152113.1 M48 family metalloprotease -
DLJ67_RS27175 101558..102812 + 1255 Protein_105 IS3 family transposase -
DLJ67_RS27180 103313..104659 - 1347 WP_020314316.1 ISNCY family transposase -
DLJ67_RS27185 104871..105353 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
DLJ67_RS27190 105341..105607 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
DLJ67_RS27195 105783..106037 - 255 WP_004152108.1 hypothetical protein -
DLJ67_RS27200 106113..106370 - 258 WP_004152107.1 hypothetical protein -
DLJ67_RS27205 106419..106622 - 204 WP_004152106.1 hemolysin expression modulator Hha -
DLJ67_RS27210 106656..107024 - 369 WP_004152105.1 hypothetical protein -
DLJ67_RS27215 107068..107562 - 495 WP_004152104.1 DNA-binding protein -
DLJ67_RS27220 107593..108168 - 576 WP_004152103.1 hypothetical protein -
DLJ67_RS27225 108156..108425 - 270 WP_004152102.1 hypothetical protein -
DLJ67_RS27230 108783..109133 + 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
DLJ67_RS27235 109183..109545 + 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mph(A) / sul1 / qacE / aadA2 / dfrA12 / blaTEM-1B / blaCTX-M-15 / aac(6')-Ib-cr / blaOXA-1 / tet(A) - 1..219996 219996


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T105968 WP_003026803.1 NZ_CP029591:c105353-104871 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T105968 NZ_CP029591:c105353-104871 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT105968 WP_003026799.1 NZ_CP029591:c105607-105341 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT105968 NZ_CP029591:c105607-105341 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References