Detailed information of TA system TA105950

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 192149..192792
• Replicon: plasmid pDA33141-217
• Accession: NZ_CP029588
• Organism: Klebsiella pneumoniae strain DA33141

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: DLJ66_RS27750
• Protein ID: WP_014386165.1
• Coordinates: 192376..192792 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: DLJ66_RS27745
• Protein ID: WP_032408893.1
• Coordinates: 192149..192379 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DLJ66_RS27715	187222..188217	-	996	WP_000611945.1	NAD(P)H-quinone oxidoreductase	-
DLJ66_RS27720	188224..189372	-	1149	WP_014386168.1	ABC transporter ATP-binding protein	-
DLJ66_RS27725	189543..190700	-	1158	WP_032408895.1	acetylornithine deacetylase	-
DLJ66_RS27730	190716..191390	-	675	WP_014386166.1	DUF1028 domain-containing protein	-
DLJ66_RS27735	191395..191829	-	435	WP_000103648.1	RidA family protein	-
DLJ66_RS27745	192149..192379	+	231	WP_032408893.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
DLJ66_RS27750	192376..192792	+	417	WP_014386165.1	PIN domain-containing protein	Toxin
DLJ66_RS27755	193115..194083	+	969	WP_074422984.1	IS5 family transposase	-
DLJ66_RS27760	194263..194637	-	375	WP_032408891.1	hypothetical protein	-
DLJ66_RS27765	194693..195019	-	327	WP_004152639.1	hypothetical protein	-
DLJ66_RS27770	195016..195744	-	729	WP_014386162.1	plasmid SOS inhibition protein A	-
DLJ66_RS27775	195741..196172	-	432	WP_014386161.1	conjugation system SOS inhibitor PsiB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / blaOXA-1 / aac(6')-Ib-cr / blaCTX-M-15 / blaTEM-1B / sul1 / qacE / aadA2 / dfrA12	-	1..216772	216772
	-	flank	IS/Tn	-	-	193115..194083	968

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-130)

Antitoxin

• SpoVT-AbrB domain (7-55)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15127.44 Da
• Isoelectric Point: 7.8841

>T105950 WP_014386165.1 NZ_CP029588:192376-192792 [Klebsiella pneumoniae]
MTKTYMLDTNICSYIMREQPEAVLKRLEQAVLRRHRIVVSAITYAEMRFGAIGKRASPRHVQLVDAFCARLDAILAWDRA
AVDATTEVKATLAAAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVAGLTFEDWVQ

Nucleotide

• Length: 417 bp

>T105950 NZ_CP029588:192376-192792 [Klebsiella pneumoniae]
ATGACCAAAACCTACATGCTGGATACTAATATCTGCTCTTATATCATGCGCGAACAACCGGAAGCGGTGCTGAAACGCCT
AGAGCAAGCGGTGTTGCGCCGTCACCGGATAGTGGTGTCAGCCATCACCTATGCCGAAATGCGTTTTGGTGCCATCGGTA
AAAGGGCTTCGCCCCGTCATGTGCAGCTGGTGGACGCATTCTGTGCGCGCCTTGATGCCATACTGGCTTGGGATCGGGCC
GCAGTTGACGCTACAACAGAGGTTAAGGCCACGCTAGCAGCTGCCGGCACGCCAATCGGCCCAAACGACACAGCCATTGC
TGGACATGCCATTGCCGCTGGCGCCATCCTGGTGACGAATAATACGCGTGAATTTGAGCGAGTCGCTGGTCTGACGTTTG
AAGACTGGGTGCAATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8854.89 Da
• Isoelectric Point: 4.5112

>AT105950 WP_032408893.1 NZ_CP029588:192149-192379 [Klebsiella pneumoniae]
MRTVSVFRNGNNRAIRLPRDLDFDGVNELEIIREGDTLILRPVKPSWSSFRQEEKADADFMAERENVVSDEGRFEL

Nucleotide

• Length: 231 bp

>AT105950 NZ_CP029588:192149-192379 [Klebsiella pneumoniae]
ATGCGAACTGTATCTGTATTCAGGAACGGTAATAACCGGGCCATCCGTCTGCCCCGCGATCTGGACTTTGACGGCGTGAA
CGAACTGGAAATCATCCGCGAGGGCGATACCCTCATCCTGCGGCCGGTAAAACCCAGTTGGAGTTCGTTCCGCCAGGAGG
AAAAAGCTGACGCAGATTTTATGGCGGAGCGTGAAAATGTTGTTAGTGACGAGGGACGTTTTGAGCTATGA