Detailed information of TA system TA105949

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 168253..168998
• Replicon: plasmid pDA33141-217
• Accession: NZ_CP029588
• Organism: Klebsiella pneumoniae strain DA33141

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A331KSM6
• Locus tag: DLJ66_RS27605
• Protein ID: WP_032408901.1
• Coordinates: 168507..168998 (+)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: DLJ66_RS27600
• Protein ID: WP_014386183.1
• Coordinates: 168253..168519 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DLJ66_RS27540	163805..164218	-	414	WP_013023817.1	helix-turn-helix domain-containing protein	-
DLJ66_RS27545	164219..164497	-	279	WP_004152721.1	helix-turn-helix domain-containing protein	-
DLJ66_RS27550	164487..164807	-	321	WP_014386190.1	type II toxin-antitoxin system RelE/ParE family toxin	-
DLJ66_RS27555	164888..165112	-	225	WP_014386189.1	hypothetical protein	-
DLJ66_RS27560	165123..165335	-	213	WP_014386188.1	hypothetical protein	-
DLJ66_RS27565	165397..165723	-	327	WP_014386187.1	hypothetical protein	-
DLJ66_RS27580	166360..166710	-	351	WP_014386186.1	hypothetical protein	-
DLJ66_RS27585	166707..166979	-	273	WP_032408902.1	hypothetical protein	-
DLJ66_RS27590	167169..167654	+	486	WP_014386185.1	hypothetical protein	-
DLJ66_RS27595	167898..168056	-	159	WP_014386184.1	type I toxin-antitoxin system Hok family toxin	-
DLJ66_RS27600	168253..168519	+	267	WP_014386183.1	DUF1778 domain-containing protein	Antitoxin
DLJ66_RS27605	168507..168998	+	492	WP_032408901.1	GNAT family N-acetyltransferase	Toxin
DLJ66_RS27610	169439..169690	-	252	WP_032408900.1	aldo/keto reductase	-
DLJ66_RS27615	169887..171479	-	1593	Protein_190	IS66 family transposase	-
DLJ66_RS27620	171510..171860	-	351	WP_014386180.1	IS66 family insertion sequence element accessory protein TnpB	-
DLJ66_RS27625	171857..172297	-	441	WP_014386179.1	IS66 family insertion sequence hypothetical protein	-
DLJ66_RS27630	172559..173314	-	756	WP_032408898.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / blaOXA-1 / aac(6')-Ib-cr / blaCTX-M-15 / blaTEM-1B / sul1 / qacE / aadA2 / dfrA12	-	1..216772	216772

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (89-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-87)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17667.46 Da
• Isoelectric Point: 8.8757

>T105949 WP_032408901.1 NZ_CP029588:168507-168998 [Klebsiella pneumoniae]
VGKVTAPVPLGTSHILAEFHCGEPVLDEWIKHRGLKNQSLGAARTFVVCRGNASQVLAYYSLATGSVTHAIAPGSLRRNM
PDPVPVIILARLAVDTSYHGQGLGADLLHDAVLRCCRVAENIGVRAVMVHALSDSARQFYIHHGFIPSQTQDRTLFLRLP
LIT

Nucleotide

• Length: 492 bp

>T105949 NZ_CP029588:168507-168998 [Klebsiella pneumoniae]
GTGGGCAAAGTAACGGCTCCGGTCCCACTCGGTACGTCACACATCCTCGCTGAGTTTCACTGTGGTGAGCCTGTACTGGA
CGAATGGATAAAGCACCGGGGACTAAAAAATCAGTCCCTGGGGGCGGCCAGAACGTTTGTGGTGTGCCGGGGAAATGCTT
CACAGGTGCTTGCCTACTACTCTCTCGCGACCGGCAGCGTCACTCATGCCATTGCGCCGGGAAGCCTGCGAAGGAATATG
CCCGATCCTGTACCTGTCATCATCCTGGCCCGTCTTGCTGTCGATACGAGTTATCACGGCCAGGGACTGGGGGCTGATTT
ACTGCATGATGCCGTATTACGTTGCTGTCGGGTGGCTGAGAACATTGGCGTGCGGGCCGTTATGGTTCATGCACTCTCTG
ACAGTGCCAGACAGTTCTACATACATCACGGATTTATCCCTTCACAAACGCAGGATAGAACCCTTTTTTTACGGTTGCCT
CTCATTACTTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10090.66 Da
• Isoelectric Point: 5.1169

>AT105949 WP_014386183.1 NZ_CP029588:168253-168519 [Klebsiella pneumoniae]
MKSDVQLNIRAKEAQRALIDTAAEILHKSRTDFILEMACQAAENVILDRRMFNLNDEQYAEFIDMLDAPVTANPAMEKLL
ARKPQWAK

Nucleotide

• Length: 267 bp

>AT105949 NZ_CP029588:168253-168519 [Klebsiella pneumoniae]
ATGAAATCTGATGTTCAGCTCAACATCCGGGCGAAAGAAGCCCAGCGGGCTCTGATTGATACTGCCGCTGAGATTCTCCA
CAAATCACGCACTGATTTTATACTGGAGATGGCCTGTCAGGCGGCGGAAAACGTCATTCTTGACCGCCGTATGTTCAATC
TCAATGATGAACAGTACGCCGAGTTTATCGACATGCTGGATGCGCCGGTCACCGCTAACCCGGCGATGGAGAAACTCCTG
GCGAGAAAACCGCAGTGGGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A331KSM6