Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 49725..50461 Replicon plasmid pDA33141-217
Accession NZ_CP029588
Organism Klebsiella pneumoniae strain DA33141

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag DLJ66_RS26850 Protein ID WP_003026803.1
Coordinates 49979..50461 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag DLJ66_RS26845 Protein ID WP_003026799.1
Coordinates 49725..49991 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DLJ66_RS26800 45787..46149 - 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -
DLJ66_RS26805 46199..46549 - 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
DLJ66_RS26810 46907..47176 + 270 WP_004152102.1 hypothetical protein -
DLJ66_RS26815 47164..47739 + 576 WP_004152103.1 hypothetical protein -
DLJ66_RS26820 47770..48264 + 495 WP_004152104.1 DNA-binding protein -
DLJ66_RS26825 48308..48676 + 369 WP_004152105.1 hypothetical protein -
DLJ66_RS26830 48710..48913 + 204 WP_004152106.1 hemolysin expression modulator Hha -
DLJ66_RS26835 48962..49219 + 258 WP_004152107.1 hypothetical protein -
DLJ66_RS26840 49295..49549 + 255 WP_004152108.1 hypothetical protein -
DLJ66_RS26845 49725..49991 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
DLJ66_RS26850 49979..50461 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
DLJ66_RS26855 50673..52019 + 1347 WP_020314316.1 ISNCY family transposase -
DLJ66_RS26860 52520..53774 - 1255 Protein_55 IS3 family transposase -
DLJ66_RS26865 53862..54824 - 963 WP_004152113.1 M48 family metalloprotease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) / blaOXA-1 / aac(6')-Ib-cr / blaCTX-M-15 / blaTEM-1B / sul1 / qacE / aadA2 / dfrA12 - 1..216772 216772


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T105948 WP_003026803.1 NZ_CP029588:49979-50461 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T105948 NZ_CP029588:49979-50461 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT105948 WP_003026799.1 NZ_CP029588:49725-49991 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT105948 NZ_CP029588:49725-49991 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References