Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 76402..77045 Replicon plasmid pDA33140-96
Accession NZ_CP029586
Organism Klebsiella pneumoniae strain DA33140

Toxin (Protein)


Gene name vagD Uniprot ID A0A7Z7T700
Locus tag DLJ83_RS28295 Protein ID WP_040155119.1
Coordinates 76402..76818 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag DLJ83_RS28300 Protein ID WP_001261276.1
Coordinates 76815..77045 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DLJ83_RS28280 71446..73554 - 2109 WP_019725134.1 peptidase domain-containing ABC transporter -
DLJ83_RS28285 73544..74821 - 1278 Protein_83 HlyD family secretion protein -
DLJ83_RS28290 75483..76361 + 879 WP_009309916.1 restriction endonuclease -
DLJ83_RS28295 76402..76818 - 417 WP_040155119.1 type II toxin-antitoxin system VapC family toxin Toxin
DLJ83_RS28300 76815..77045 - 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
DLJ83_RS28315 77960..78268 + 309 WP_004152336.1 hypothetical protein -
DLJ83_RS28320 78296..78625 + 330 WP_004152337.1 hypothetical protein -
DLJ83_RS28325 78651..79049 + 399 WP_004171440.1 hypothetical protein -
DLJ83_RS28330 79056..79388 + 333 WP_004152339.1 hypothetical protein -
DLJ83_RS28335 79388..80170 + 783 WP_004152340.1 site-specific integrase -
DLJ83_RS28340 81062..81292 - 231 WP_011977773.1 hypothetical protein -
DLJ83_RS28345 81384..81857 - 474 WP_004152341.1 YkgJ family cysteine cluster protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid aph(3'')-Ib / aph(6)-Id / aac(6')-Ib-cr / blaOXA-1 / aac(3)-IIa / blaCTX-M-15 / blaTEM-1B / qnrS1 / sul1 / qacE / dfrA1 / tet(A) / catA1 - 1..96190 96190
- flank IS/Tn - - 81977..83245 1268


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15147.60 Da        Isoelectric Point: 7.8915

>T105930 WP_040155119.1 NZ_CP029586:c76818-76402 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPESVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIHLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFERVPGLVLEDWVK

Download         Length: 417 bp

>T105930 NZ_CP029586:c76818-76402 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGAATCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGACCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCATCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCGTCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCGGCGGGCGCCATCCTGGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT105930 WP_001261276.1 NZ_CP029586:c77045-76815 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT105930 NZ_CP029586:c77045-76815 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGATGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7Z7T700

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References