Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2138442..2139067 Replicon chromosome
Accession NZ_CP029582
Organism Klebsiella pneumoniae strain DA33140

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag DLJ83_RS10550 Protein ID WP_002882817.1
Coordinates 2138684..2139067 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag DLJ83_RS10545 Protein ID WP_004150355.1
Coordinates 2138442..2138684 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DLJ83_RS10520 2134433..2135032 + 600 WP_004151865.1 glucose-1-phosphatase -
DLJ83_RS10525 2135026..2135886 + 861 WP_004146232.1 virulence factor BrkB family protein -
DLJ83_RS10530 2135883..2136320 + 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
DLJ83_RS10535 2136365..2137306 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
DLJ83_RS10540 2137320..2138237 - 918 WP_021466676.1 alpha/beta hydrolase -
DLJ83_RS10545 2138442..2138684 + 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
DLJ83_RS10550 2138684..2139067 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
DLJ83_RS10555 2139241..2140170 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
DLJ83_RS10560 2140167..2140802 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
DLJ83_RS10565 2140799..2141701 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T105915 WP_002882817.1 NZ_CP029582:2138684-2139067 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T105915 NZ_CP029582:2138684-2139067 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT105915 WP_004150355.1 NZ_CP029582:2138442-2138684 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT105915 NZ_CP029582:2138442-2138684 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References