Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 551533..552269 Replicon chromosome
Accession NZ_CP029582
Organism Klebsiella pneumoniae strain DA33140

Toxin (Protein)


Gene name tacT Uniprot ID A0A4S4Y1H2
Locus tag DLJ83_RS02570 Protein ID WP_032433360.1
Coordinates 551533..552015 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag DLJ83_RS02575 Protein ID WP_003026799.1
Coordinates 552003..552269 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DLJ83_RS02550 546688..547287 + 600 WP_032432064.1 helix-turn-helix transcriptional regulator -
DLJ83_RS02555 547667..548299 + 633 WP_001567369.1 hypothetical protein -
DLJ83_RS02560 548328..549731 - 1404 WP_039815096.1 ISNCY-like element ISKpn21 family transposase -
DLJ83_RS02565 549843..551375 + 1533 WP_015065592.1 IS3-like element ISKpn38 family transposase -
DLJ83_RS02570 551533..552015 - 483 WP_032433360.1 GNAT family N-acetyltransferase Toxin
DLJ83_RS02575 552003..552269 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
DLJ83_RS02580 552582..553145 - 564 WP_032433361.1 6-phospho-3-hexuloisomerase -
DLJ83_RS02585 553155..553787 - 633 WP_032433362.1 3-hexulose-6-phosphate synthase -
DLJ83_RS02590 553805..554809 - 1005 WP_032433364.1 dihydroxyacetone kinase subunit DhaK -
DLJ83_RS02595 554793..555455 - 663 WP_032433366.1 dihydroxyacetone kinase subunit L -
DLJ83_RS02600 555484..556623 - 1140 WP_032433368.1 mannitol dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 536705..584324 47619


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(72-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17421.02 Da        Isoelectric Point: 9.6047

>T105910 WP_032433360.1 NZ_CP029582:c552015-551533 [Klebsiella pneumoniae]
VGRVTAPEPLSSSHQLAEFVSGETVLDEWLKHRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTETTGRLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYTHHGFTASQTHERTLFLKLP

Download         Length: 483 bp

>T105910 NZ_CP029582:c552015-551533 [Klebsiella pneumoniae]
GTGGGACGTGTAACCGCGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTTGA
TGAATGGCTAAAGCACAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGGACGA
AGCAAGTAGCTGGTTTTTACTCTCTGGCCACCGGTAGCGTTAACCATACGGAAACTACAGGCAGACTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCCGTTCTACGCTGTTATCGCGTAGCCGAGAATATTGGCGTCCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCTAAAGGCTTCTATACTCACCATGGATTTACGGCATCGCAAACTCATGAGCGGACTCTGTTCCTTAAACTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT105910 WP_003026799.1 NZ_CP029582:c552269-552003 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT105910 NZ_CP029582:c552269-552003 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCTTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4S4Y1H2

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References