Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 33350..33951 Replicon plasmid pDA33137-178
Accession NZ_CP029580
Organism Escherichia coli strain DA33137

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag DLJ64_RS27420 Protein ID WP_001216034.1
Coordinates 33571..33951 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag DLJ64_RS27415 Protein ID WP_001190712.1
Coordinates 33350..33571 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DLJ64_RS27405 30331..31614 - 1284 WP_001617890.1 restriction endonuclease subunit S -
DLJ64_RS27410 31611..33167 - 1557 WP_001617892.1 type I restriction-modification system subunit M -
DLJ64_RS27415 33350..33571 + 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
DLJ64_RS27420 33571..33951 + 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
DLJ64_RS27425 33956..34135 + 180 WP_001513661.1 hypothetical protein -
DLJ64_RS27430 34163..34522 + 360 WP_001513660.1 hypothetical protein -
DLJ64_RS27435 34446..34859 + 414 Protein_39 DDE-type integrase/transposase/recombinase -
DLJ64_RS27440 34809..35126 - 318 WP_001513659.1 hypothetical protein -
DLJ64_RS27450 35198..35895 + 698 Protein_41 IS1 family transposase -
DLJ64_RS27455 36131..37111 - 981 WP_000019407.1 IS5-like element IS5 family transposase -
DLJ64_RS27460 37355..38758 + 1404 WP_001373486.1 S-methylmethionine permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid qacE / blaCTX-M-14 / aadA5 / sul1 / mph(A) / erm(B) / aac(3)-IId / blaTEM-1B / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / cmlA1 senB 1..178078 178078
- inside Integron - senB 27..178076 178049


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T105901 WP_001216034.1 NZ_CP029580:33571-33951 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T105901 NZ_CP029580:33571-33951 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT105901 WP_001190712.1 NZ_CP029580:33350-33571 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT105901 NZ_CP029580:33350-33571 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References