Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 22881..23406 Replicon plasmid pDA33137-178
Accession NZ_CP029580
Organism Escherichia coli strain DA33137

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag DLJ64_RS27370 Protein ID WP_001159868.1
Coordinates 22881..23186 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag DLJ64_RS27375 Protein ID WP_000813634.1
Coordinates 23188..23406 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DLJ64_RS27355 18845..20011 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
DLJ64_RS27360 20599..21354 - 756 WP_000852146.1 replication initiation protein RepE -
DLJ64_RS27365 22074..22880 - 807 WP_000016982.1 site-specific integrase -
DLJ64_RS27370 22881..23186 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
DLJ64_RS27375 23188..23406 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
DLJ64_RS27380 24114..25109 + 996 WP_000246636.1 hypothetical protein -
DLJ64_RS27385 25113..26045 + 933 WP_000991832.1 hypothetical protein -
DLJ64_RS27390 26182..26879 - 698 Protein_31 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 26182..26685 503
- inside Integron - senB 27..178076 178049
- inside Conjugative plasmid qacE / blaCTX-M-14 / aadA5 / sul1 / mph(A) / erm(B) / aac(3)-IId / blaTEM-1B / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / cmlA1 senB 1..178078 178078


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T105900 WP_001159868.1 NZ_CP029580:c23186-22881 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T105900 NZ_CP029580:c23186-22881 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT105900 WP_000813634.1 NZ_CP029580:c23406-23188 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT105900 NZ_CP029580:c23406-23188 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References