Detailed information of TA system TA105899

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 5144598..5145236
• Replicon: chromosome
• Accession: NZ_CP029579
• Organism: Escherichia coli strain DA33137

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0D8WF76
• Locus tag: DLJ64_RS26605
• Protein ID: WP_001306887.1
• Coordinates: 5144598..5144774 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: DLJ64_RS26610
• Protein ID: WP_001270286.1
• Coordinates: 5144820..5145236 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DLJ64_RS26580	5139654..5140112	+	459	WP_000526135.1	IS200/IS605 family transposase	-
DLJ64_RS26585	5140220..5141431	-	1212	WP_023143671.1	BenE family transporter YdcO	-
DLJ64_RS26590	5141484..5142020	+	537	WP_020233877.1	DNA-binding transcriptional regulator SutR	-
DLJ64_RS26595	5142093..5144054	+	1962	WP_024166512.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
DLJ64_RS26600	5144146..5144376	-	231	WP_000494241.1	YncJ family protein	-
DLJ64_RS26605	5144598..5144774	+	177	WP_001306887.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
DLJ64_RS26610	5144820..5145236	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
DLJ64_RS26615	5145315..5146721	+	1407	WP_001523229.1	PLP-dependent aminotransferase family protein	-
DLJ64_RS26620	5146966..5148111	+	1146	WP_020233879.1	ABC transporter substrate-binding protein	-
DLJ64_RS26625	5148129..5149142	+	1014	WP_001523231.1	ABC transporter ATP-binding protein	-
DLJ64_RS26630	5149143..5150084	+	942	WP_001251315.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	5139654..5140112	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6768.88 Da
• Isoelectric Point: 11.5336

>T105899 WP_001306887.1 NZ_CP029579:5144598-5144774 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFRGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T105899 NZ_CP029579:5144598-5144774 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCGTGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT105899 WP_001270286.1 NZ_CP029579:5144820-5145236 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT105899 NZ_CP029579:5144820-5145236 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACAGTTGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGACGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAATTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A0D8WF76