Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3611002..3611656 Replicon chromosome
Accession NZ_CP029579
Organism Escherichia coli strain DA33137

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag DLJ64_RS18795 Protein ID WP_000244781.1
Coordinates 3611249..3611656 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag DLJ64_RS18790 Protein ID WP_000354046.1
Coordinates 3611002..3611268 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DLJ64_RS18770 3607090..3608523 - 1434 WP_001521235.1 6-phospho-beta-glucosidase BglA -
DLJ64_RS18775 3608568..3608879 + 312 WP_001182949.1 N(4)-acetylcytidine aminohydrolase -
DLJ64_RS18780 3609043..3609702 + 660 WP_000250269.1 hemolysin III family protein -
DLJ64_RS18785 3609779..3610759 - 981 WP_000886050.1 tRNA-modifying protein YgfZ -
DLJ64_RS18790 3611002..3611268 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
DLJ64_RS18795 3611249..3611656 + 408 WP_000244781.1 protein YgfX Toxin
DLJ64_RS18800 3611696..3612217 - 522 WP_001521233.1 flavodoxin FldB -
DLJ64_RS18805 3612329..3613225 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
DLJ64_RS18810 3613250..3613960 + 711 WP_001521231.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
DLJ64_RS18815 3613966..3615699 + 1734 WP_021523238.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T105889 WP_000244781.1 NZ_CP029579:3611249-3611656 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T105889 NZ_CP029579:3611249-3611656 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATCTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATCCACGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGCTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT105889 WP_000354046.1 NZ_CP029579:3611002-3611268 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT105889 NZ_CP029579:3611002-3611268 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAGTGTGACGATCCCGATC
TGTTTAACTGGCTGATGAACCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References